EMPIAR-10874 - Single-Particle CryoEM of mammalian V-ATPase with the TLDc domain protein mEAK7 bound (Various Datasets) [multiple data sets in MRCS, MRC and EER formats]

EMPIAR-10874

Single-Particle CryoEM of mammalian V-ATPase with the TLDc domain protein mEAK7 bound (Various Datasets) [multiple data sets in MRCS, MRC and EER formats]

Publication:

CryoEM of endogenous mammalian V-ATPase interacting with the TLDc protein mEAK-7

Tan YZ, Abbas YM, Wu JZ, Wu D, Keon KA, Hesketh GG, Bueler SA, Gingras AC, Robinson CV, Grinstein S, Rubinstein JL

Life science alliance 5 (2022)

PMID: 35794005

DOI: 10.26508/lsa.202201527

Related EMDB entry:

EMD-26386 EMD-26385 EMD-26387 EMD-26388

Deposited:

2021-11-08

Released:

2022-09-05

Last modified:

2022-09-05

Imageset size:

12.71 TB

Imageset DOI:

10.6019/EMPIAR-10874

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
micrographs - single frame
picked particles - single frame - processed
picked particles - multiframe - processed

Image Sets

1. Unaligned multiframe movies of Pig Kidney V-ATPase bound to mEAK-7 collected using Tundra

Category:

micrographs - multiframe

Image format:

MRCS

No. of images or tilt series:

1957

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.7512, 0.7512)

Details:

Movies collected using Tundra. 24 frames were used, pixel size is 0.7512 Å/pixel, total dose of 40 e-/Å2. Compressed using pbzip2.

Files:

Archives and downloads the selected files into an uncompressed zip file.

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2. Aligned and dose-weighted micrographs of Pig Kidney V-ATPase bound to mEAK-7 collected using Tundra

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

1957

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.7512, 0.7512)

Details:

Micrographs collected using Tundra. Aligned and dose weighted using UCSF MotionCor2.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Polished particles of Pig Kidney V-ATPase bound to mEAK-7 collected using Tundra

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

Image size:

(320, 320)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.461, 1.461)

Details:

Final particle stack after particle polishing using Relion.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

4. Unaligned multiframe movies of Pig Kidney V-ATPase bound to mEAK-7 collected using Titan Krios and Falcon4

Category:

micrographs - multiframe

Image format:

MRCS

No. of images or tilt series:

5142

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.3225, 1.3225)

Details:

Movies collected using Titan Krios and Falcon 4. 30 frames were used, pixel size is 1.3225 Å/pixel, total dose of 36 e-/Å2. Compressed using pbzip2. Last frame should be discarded during processing.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

5. Aligned and dose-weighted micrographs of Pig Kidney V-ATPase bound to mEAK-7 collected using Titan Krios and Falcon4

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

5142

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.3225, 1.3225)

Details:

Micrographs collected using Titan Krios and Falcon 4. Aligned and dose weighted using UCSF MotionCor2.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

6. Polished particles of Pig Kidney V-ATPase bound to mEAK-7 collected using Titan Krios and Falcon4

Category:

picked particles - multiframe - processed

Image format:

MRCS

No. of images or tilt series:

Image size:

(360, 360)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.3225, 1.3225)

Details:

Final particle stack after particle polishing using Relion.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

7. Unaligned multiframe movies of Pig Kidney V-ATPase bound to mEAK-7deltaCterm collected using Titan Krios and Falcon4

Category:

micrographs - multiframe

Image format:

MRCS

No. of images or tilt series:

2013

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.3225, 1.3225)

Details:

Movies collected using Titan Krios and Falcon 4. 30 frames were used, pixel size is 1.3225 Å/pixel, total dose of 37 e-/Å2. Compressed using pbzip2. Last frame should be discarded during processing.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

8. Aligned and dose-weighted micrographs of Pig Kidney V-ATPase bound to mEAK-7deltaCterm collected using Titan Krios and Falcon4

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

2013

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.3225, 1.3225)

Details:

Micrographs collected using Titan Krios and Falcon 4. Aligned and dose weighted using UCSF MotionCor2.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

9. Polished particles of Pig Kidney V-ATPase bound to mEAK-7deltaCterm collected using Titan Krios and Falcon4

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.3225, 1.3225)

Details:

Final particle stack after particle polishing using Relion.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

10. Unaligned multiframe movies of Pig Kidney V-ATPase bound to mEAK-7 with ATP collected using Titan Krios and Falcon4

Category:

micrographs - multiframe

Image format:

MRCS

No. of images or tilt series:

5498

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.3225, 1.3225)

Details:

Movies collected using Titan Krios and Falcon 4. 30 frames were used, pixel size is 1.3225 Å/pixel, total dose of 44 e-/Å2. Compressed using pbzip2. Last frame should be discarded during processing.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

11. Aligned and dose-weighted micrographs of Pig Kidney V-ATPase bound to mEAK-7 with ATP collected using Titan Krios and Falcon4

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

5498

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.3225, 1.3225)

Details:

Micrographs collected using Titan Krios and Falcon 4. Aligned and dose weighted using UCSF MotionCor2.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

12. Polished particles of Pig Kidney V-ATPase bound to mEAK-7 with ATP collected using Titan Krios and Falcon4

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.3225, 1.3225)

Details:

Final particle stack after particle polishing using Relion.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

13. Unaligned multiframe movies of Pig Kidney V-ATPase bound to mEAK-7 with EDTA/EGTA collected using Titan Krios and Falcon4

Category:

micrographs - multiframe

Image format:

MRCS

No. of images or tilt series:

2468

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.3225, 1.3225)

Details:

Movies collected using Titan Krios and Falcon 4. 30 frames were used, pixel size is 1.3225 Å/pixel, total dose of 41 e-/Å2. Compressed using pbzip2. Last frame should be discarded during processing.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

14. Aligned and dose-weighted micrographs of Pig Kidney V-ATPase bound to mEAK-7 with EDTA/EGTA collected using Titan Krios and Falcon4

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

2468

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.3225, 1.3225)

Details:

Micrographs collected using Titan Krios and Falcon 4. Aligned and dose weighted using UCSF MotionCor2.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

15. Polished particles of Pig Kidney V-ATPase bound to mEAK-7 with EDTA/EGTA collected using Titan Krios and Falcon4

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

Image size:

(360, 360)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.3225, 1.3225)

Details:

Final particle stack after particle polishing using Relion.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

16. Unaligned multiframe movies of Pig Kidney V-ATPase bound to mEAK-7 with Calcium collected using Glacios with Selectris X and Falcon 4

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

3029

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.895, 0.895)

Details:

Movies collected using Glacios with Selectris X and Falcon 4. EER format was used, pixel size is 0.895 Å/pixel, total dose of 40 e-/Å2.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

17. Aligned and dose-weighted micrographs of Pig Kidney V-ATPase bound to mEAK-7 with Calcium collected using Glacios with Selectris X and Falcon 4

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

3029

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.895, 0.895)

Details:

Micrographs collected using Titan Krios and Falcon 4. Aligned and dose weighted using UCSF MotionCor2.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

18. Polished particles of Pig Kidney V-ATPase bound to mEAK-7 with Calcium collected using Glacios with Selectris X and Falcon 4

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

Image size:

(320, 320)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.56625, 1.56625)

Details:

Final particle stack after particle polishing using Relion.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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