EMPIAR-10824 - Cryo electron micrographs of Pol delta-DNA-PCNA giving rise to various PCNA tilt angles [multiple data sets in MRC format]

EMPIAR-10824

Cryo electron micrographs of Pol delta-DNA-PCNA giving rise to various PCNA tilt angles [multiple data sets in MRC format]

Publication:

Structure of the processive human Pol δ holoenzyme

Lancey C, Tehseen M, Raducanu VS, Rashid F, Merino N, Ragan TJ, Savva CG, Zaher MS, Shirbini A, Blanco FJ, Hamdan SM, De Biasio A

Nature communications 11 (2020)

PMID: 32111820

DOI: 10.1038/s41467-020-14898-6

Related EMDB entry:

EMD-10081 EMD-10080 EMD-10082

Deposited:

2021-09-15

Released:

2021-10-26

Last modified:

2021-10-26

Imageset size:

4.01 TB

Imageset DOI:

10.6019/EMPIAR-10824

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Cryo-electron micrographs of Pol delta holoenzyme giving rise to different tilted conformers, collection 2

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

1406

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(1.081, 1.081)

Details:

Images taken on a FEI Titan Krios G3 at 300kV with a Falcon 3 detector. Defocus -0.6 micrometers. Volta phase plate was used. Total dose = 36 e-/Å^2. Dataset includes 3 corrupted files, as file names suggest - these became corrupted after the deposited EMDB maps were obtained.

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2. Cryo-electron micrographs of Pol delta holoenzyme giving rise to different tilted conformers, collection 1

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

452

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(1.081, 1.081)

Details:

Images taken on a FEI Titan Krios G3 at 300kV with a Falcon 3 detector. Defocus -0.4 micrometers. Volta phase plate was used. Total dose = 36 e-/Å^2. Dataset includes 2 corrupted files, as file names suggest - these became corrupted after the deposited EMDB maps were obtained.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Cryo-electron micrographs of Pol delta holoenzyme giving rise to different tilted conformers, collection 3

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

1717

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(1.081, 1.081)

Details:

Images taken on a FEI Titan Krios G3 at 300kV with a Falcon 3 detector. Defocus -0.6 micrometers. Volta phase plate was used. Total dose = 36 e-/Å^2.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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