Each subdirectory with 12 random characters contains a single 2D classification run, with an image file run_class.mrcs that contains the actual 2D class averages, the files run_model.star, run_data.star, run_sampling.star and run_optimiser.star with the corresponding metadata from RELION's 2D classification run (see RELION documentation for details), a file job_score.txt that contains the manually assigned job score for that 2D classification run, a backup_selection.star file that contains the different categories of assigned classes, which are converted to individual class scores in the class_ranker program (see function ClassRanker::getClassScoreFromJobScore inside src/class_ranker.cpp), and a file features_normalized.star that contains the features calculated by the region_class_ranker program.
One can visualise the images for each class, e.g. in directory cahg4Zo4Goos, with the following command:
Classes shown in red (1 in backup_selection.star) are the best according to the manually assigned class labels in backup_selection.star; magenta (5) are second-best; green (2) third-best; and blue (3) or cyan (4) fourth-best. Yellow classes (6) or non-coloured classes (0) are the worst (score=0).
The normalised_features.star file was calculated in RELION-4.0, running the following command in csh from the main directory:
Archives and downloads the selected files into an uncompressed zip file.
Depending on the number and size of files to be downloaded, this can take an enormous amount of time.
Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.
We recommend using rsync, aspera, globus, etc.
Download a list of selected files.
It is possible to download files by specifying the file list with rsync command, etc.
Archives and downloads the selected files into an uncompressed zip file.
Depending on the number and size of files to be downloaded, this can take an enormous amount of time.
Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.
We recommend using rsync, aspera, globus, etc.
Download a list of selected files.
It is possible to download files by specifying the file list with rsync command, etc.