EMPIAR-10736 - Cryo-EM of the human insulin receptor ectodomain in complex with an insulin analog with truncated B chain and enlongated A chain [multiple data sets in MRC format]

EMPIAR-10736

Cryo-EM of the human insulin receptor ectodomain in complex with an insulin analog with truncated B chain and enlongated A chain [multiple data sets in MRC format]

Publication:

Symmetric and asymmetric receptor conformation continuum induced by a new insulin

Forbes BE, Safavi-Hemami H, Hill CP, Chou DH

Nature chemical biology 18 (2022) 511-519

PMID: 35289328

DOI: 10.1038/s41589-022-00981-0

Related EMDB entry:

EMD-23950 EMD-23951 EMD-23949

Deposited:

2021-05-12

Released:

2022-03-14

Last modified:

2022-09-15

Imageset size:

2.41 TB

Imageset DOI:

10.6019/EMPIAR-10736

Experimental metadata:

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Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

Movies in this entry were compressed to TIFF by EMPIAR Japan to save space and bandwidth.
The compression is lossless in that all pixel intensities remain exactly the same after decompression.
However, some metadata in the MRC header were lost.
The original files before compression are available from EMPIAR EBI.

The following three files were found corrupted and removed.

- movies-dose-60/FoilHole_22055695_Data_22053810_22053812_20191121_2111-86724.mrc
- movies-dose-60/FoilHole_22055696_Data_22053772_22053774_20191121_2113-86730.mrc
- movies-dose-60/FoilHole_22055696_Data_22053778_22053780_20191121_2113-86731.mrc

Original size:   12,569,361,786 kilobytes
Compressed size:  2,357,786,229 kilobytes

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs (40 e-/A2 dose)

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

7221

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.365, 1.365)

Details:

Raw, uncurated cryo-EM movies. Not gain corrected. Use gain image matching pattern *-gain-ref.mrc

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2. unaligned micrographs (60 e-/A2 dose)

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

4078

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.365, 1.365)

Details:

Raw, uncurated cryo-EM movies. Not gain corrected. Use gain image matching pattern *-gain-ref.mrc

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

EMICSS: added-value annotations for EMDB entries.

Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)

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