EMPIAR-10734 - Cryo electron microscopy of ex-vivo human SAA amyloid fibrils [6465 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-10734

Cryo electron microscopy of ex-vivo human SAA amyloid fibrils [6465 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Cryo-EM fibril structures from systemic AA amyloidosis reveal the species complementarity of pathological amyloids

Liberta F, Loerch S, Rennegarbe M, Schierhorn A, Westermark P, Westermark GT, Hazenberg BPC, Grigorieff N, Fändrich M, Schmidt M

Nature communications 10 (2019)

PMID: 30846696

DOI: 10.1038/s41467-019-09033-z

Related EMDB entry:

EMD-9232

Deposited:

2021-06-16

Released:

2021-09-01

Last modified:

2021-09-01

Imageset size:

1.09 TB

Imageset DOI:

10.6019/EMPIAR-10734

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multiframe micrographs of ex-vivo human SAA1

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

6465

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.04, 1.04)

Details:

Data collection with a Titan Krios EM (300 kV) using a K2 Summit camera. Defocus range -0.5 to -2.5, exposure time 12 seconds, dose rate 3.33 e-/Å^2/s, total dose 40 e-/Å^2.
For CTF calculations: CS = 2.7 mm, HT = 300 kV, AmpCnst = 0.07, XMAG = 48076.9, DStep = 50000 um

Thumbnails and powerspectra of ex-vivo human SAA1 micrographs are also provided, as well as the gain reference.

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