EMPIAR-10718
The structure of human CST reveals a decameric assembly bound to telomeric DNA [multiple data sets in TIFF and MRC formats]
Publication:

The structure of human CST reveals a decameric assembly bound to telomeric DNA

Lim C, Barbour AT, Zaug AJ, Goodrich KJ, McKay AE, Wuttke DS, Cech TR

Science (New York, N.Y.) 368 (2020) 1081-1085

PMID: 32499435

Related EMDB entry:
Deposited:
2021-05-17
Released:
2021-06-25
Last modified:
2021-06-25
Imageset size:
14.25 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • micrographs - single frame
1. Unaligned movies of CST with 3xTEL DNA oligomer at 0 degrees stage tilt
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
20808
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.539, None)
Details:
This dataset was collected on a KRIOS with 3x3 beam tilt strategy using SerialEM. The optical groups are named just before the date. For example, W3_3_999_009_Aug02_00.49.32.tif is group 9. In addition, this dataset was collected at 0 degrees stage tilt with a pixel size of 0.539 Å/pixel, a defocus range of -1 to -2.5 µm, and a total dose of 60.0 e-/Å2 (60 frames) using a Gatan K3 direct electron detector in super-resolution CDS mode. Gain reference to be applied.
Files:
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2. Unaligned movies of CST with 3xTEL DNA oligomer at 30 degrees stage tilt
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
7227
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.539, None)
Details:
This dataset was collected on a KRIOS with 3x3 beam tilt strategy using SerialEM. The optical groups are named just before the date. For example, W3_3_30tilt_3315_004_Aug08_10.16.04.tif is group 4. In addition, this dataset was collected at 30 degrees stage tilt with a pixel size of 0.539 Å/pixel, a defocus range of -1 to -2.5 µm, and a total dose of 60.0 e-/Å2 (60 frames) using a Gatan K3 direct electron detector in super-resolution CDS mode. Gain reference to be applied.
Files:
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3. Gain reference for both datasets - gainref_20190729.mrc
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
1
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.539, None)
Details:
Gain reference for the two datasets.
Files:
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Files:
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