EMPIAR-10718 - The structure of human CST reveals a decameric assembly bound to telomeric DNA [multiple data sets in TIFF and MRC formats]

EMPIAR-10718

The structure of human CST reveals a decameric assembly bound to telomeric DNA [multiple data sets in TIFF and MRC formats]

Publication:

The structure of human CST reveals a decameric assembly bound to telomeric DNA

Lim C, Barbour AT, Zaug AJ, Goodrich KJ, McKay AE, Wuttke DS, Cech TR

Science (New York, N.Y.) 368 (2020) 1081-1085

PMID: 32499435

DOI: 10.1126/science.aaz9649

Related EMDB entry:

EMD-21567

Deposited:

2021-05-17

Released:

2021-06-25

Last modified:

2021-06-25

Imageset size:

14.25 TB

Imageset DOI:

10.6019/EMPIAR-10718

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
micrographs - single frame

Image Sets

1. Unaligned movies of CST with 3xTEL DNA oligomer at 0 degrees stage tilt

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

20808

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.539, None)

Details:

This dataset was collected on a KRIOS with 3x3 beam tilt strategy using SerialEM. The optical groups are named just before the date. For example, W3_3_999_009_Aug02_00.49.32.tif is group 9. In addition, this dataset was collected at 0 degrees stage tilt with a pixel size of 0.539 Å/pixel, a defocus range of -1 to -2.5 µm, and a total dose of 60.0 e-/Å2 (60 frames) using a Gatan K3 direct electron detector in super-resolution CDS mode. Gain reference to be applied.

Files:

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2. Unaligned movies of CST with 3xTEL DNA oligomer at 30 degrees stage tilt

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

7227

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.539, None)

Details:

This dataset was collected on a KRIOS with 3x3 beam tilt strategy using SerialEM. The optical groups are named just before the date. For example, W3_3_30tilt_3315_004_Aug08_10.16.04.tif is group 4. In addition, this dataset was collected at 30 degrees stage tilt with a pixel size of 0.539 Å/pixel, a defocus range of -1 to -2.5 µm, and a total dose of 60.0 e-/Å2 (60 frames) using a Gatan K3 direct electron detector in super-resolution CDS mode. Gain reference to be applied.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Gain reference for both datasets - gainref_20190729.mrc

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.539, None)

Details:

Gain reference for the two datasets.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

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