EMPIAR-10697 - Cryo-EM structures of human RNA Polymerase III [multiple data sets in TIFF and MRCS formats]

EMPIAR-10697

Cryo-EM structures of human RNA Polymerase III [multiple data sets in TIFF and MRCS formats]

Publication:

Cryo-EM structures of human RNA polymerase III in its unbound and transcribing states

Girbig M, Misiaszek AD, Vorländer MK, Lafita A, Grötsch H, Baudin F, Bateman A, Müller CW

Nature structural & molecular biology 28 (2021) 210-219

PMID: 33558764

DOI: 10.1038/s41594-020-00555-5

Related EMDB entry:

EMD-11742 EMD-11736 EMD-11738 EMD-11673 EMD-11740 EMD-11737 EMD-11739 EMD-11741

Deposited:

2020-10-21

Released:

2021-05-07

Last modified:

2021-05-07

Imageset size:

3.27 TB

Imageset DOI:

10.6019/EMPIAR-10697

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
picked particles - single frame - processed

Image Sets

1. LZW-TIFF compressed multiframe micrographs of human RNA Pol III EC

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

9488

Image size:

(3710, 3838)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.05, 1.05)

Details:

Micrographs were recorded in counting mode, dose = 1.01 e/A^2/frame
Defocus range = 0.75 - 2.00
CountRef_41-1_00001.mrc to be found in "data/EMPIAR/Human_Pol_III_EC/01_raw_tiffs/"

Files:

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2. Initially picked particles of human RNA Pol III EC

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

367717

Image size:

(250, 250)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.05, 1.05)

Details:

Directory "data/EMPIAR/Human_Pol_III_EC/02__initially_picked_particles" also contains the "run_data.star" file, which contains the coordinates of the particles that went into the final 3D reconstruction of human Pol III EC (unpolished)

Files:

Archives and downloads the selected files into an uncompressed zip file.

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rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Polished particles of human RNA Pol III EC (final reconstruction)

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

166071

Image size:

(440, 440)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.05, 1.05)

Details:

Directory "data/EMPIAR/Human_Pol_III_EC/03_polished_particles_map_B" contains the "run_data.star" file of the final 3D reconstruction derived from Relion 3D Refinement after particle polishing and CTF refinement

Files:

Archives and downloads the selected files into an uncompressed zip file.

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4. LZW-TIFF compressed multiframe micrographs of human RNA Pol III apo

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

9544

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.822, 0.822)

Details:

Micrographs were recorded in counting mode, dose = 0.95 e/A^2/frame
Defocus range = 0.75 - 2.25
CountRef_51-1_00001.mrc to be found in "data/EMPIAR/Human_Pol_III_apo/01_raw_tiffs/"

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

5. Initially picked particles of human RNA Pol III apo

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

304683

Image size:

(328, 328)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.822, 0.822)

Details:

Directory "data/EMPIAR/Human_Pol_III_apo/02_initially_picked_particles" also contains the "run_data.star" file, which contains the coordinates of the particles that went into the final 3D reconstruction of human Pol III apo (unpolished)

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

6. Polished particles of human RNA Pol III apo (final reconstruction)

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

111289

Image size:

(440, 440)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.822, 0.822)

Details:

Directory "data/EMPIAR/Human_Pol_III_apo/03_polished_particles_map_A" contains the "run_data.star" file of the final 3D reconstruction derived from Relion 3D Refinement after particle polishing and CTF refinement

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Files:

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It is possible to download files by specifying the file list with rsync command, etc.

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