EMPIAR-10689 - Cryo-electron tomography of FIB-milled Caulobacter crescentus expressing PopZ with IDR-156 and pentavalent OD [multiple data sets in MRC and TIFF formats]

EMPIAR-10689

Cryo-electron tomography of FIB-milled Caulobacter crescentus expressing PopZ with IDR-156 and pentavalent OD [multiple data sets in MRC and TIFF formats]

Publication:

The material properties of a bacterial-derived biomolecular condensate tune biological function in natural and synthetic systems

Lasker K, Boeynaems S, Lam V, Scholl D, Stainton E, Briner A, Jacquemyn M, Daelemans D, Deniz A, Villa E, Holehouse AS, Gitler AD, Shapiro L

Nature communications 13 (2022)

PMID: 36163138

DOI: 10.1038/s41467-022-33221-z

Related EMDB entry:

EMD-23624

Deposited:

2021-04-07

Released:

2023-01-16

Last modified:

2023-01-16

Imageset size:

5.92 GB

Imageset DOI:

10.6019/EMPIAR-10689

Experimental metadata:

Download xml json

Contains:

tilt series
micrographs - multiframe

Image Sets

1. Unaligned tilt series of FIB-milled Caulobacter crescentus expressing PopZ with IDR-156 and pentavalent OD

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(3710, 3838)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(4.265, 4.265)

Details:

Tilt-series is motion-corrected using Warp. This tilt-series was generated with the first three frames of each tilt micrograph removed.

Files:

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2. Multi-frame micrographs of each tilt for tilt-series of FIB-milled Caulobacter crescentus expressing PopZ with IDR-156 and pentavalent OD

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

Image size:

(3710, 3838)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(4.265, 4.265)

Details:

Dark-subtracted frames from serialEM. mdoc metadata file and counting mode gain-reference is included in the same directory.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

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