EMPIAR-10669
Cryo-EM dataset of the substrate-engaged human 26S proteasome [44688 micrographs in MRC format]
Publication:

Cryo-EM structures and dynamics of substrate-engaged human 26S proteasome

Dong Y, Zhang S, Wu Z, Li X, Wang WL, Zhu Y, Stoilova-McPhie S, Lu Y, Finley D, Mao Y

Nature 565 (2018) 49-55

PMID: 30479383

Deposited:
2020-12-23
Released:
2021-11-30
Last modified:
2021-11-30
Imageset size:
13.95 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - single frame
1. Drift-corrected frame-averaged super-counting mode micrographs and extracted particles of substrate-engaged human 26S proteasome
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
44688
Image size:
(7676, 7420)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.685, 0.685)
Details:
The micrographs were the drift-corrected averages of 39 raw move frames, accumulating 44 electron per squared Angstrom in a 10 second exposure, which were taken under super-counting mode of K2 Summit camera. The original movie contains 40 frames, with the first frame not being used after drift correction for the averaged micrographs. The original particle set picked and selected with removal of apparent junks is specified in data/particles/26S-original.star, and the remaining E*.star files specify the final homogeneous datasets of RP-CP states classified by AlphaCryo4D.
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