EMPIAR-10665 - Single particle Cryo-EM data set for study the structural basis of Phosphatidylinositol 3-kinase type 2α (PI3KC2α) [multiple data sets in MRC format]

EMPIAR-10665

Single particle Cryo-EM data set for study the structural basis of Phosphatidylinositol 3-kinase type 2α (PI3KC2α) [multiple data sets in MRC format]

Publication:

Structural basis of phosphatidylinositol 3-kinase C2α function

Lo WT, Zhang Y, Vadas O, Roske Y, Gulluni F, De Santis MC, Zagar AV, Stephanowitz H, Hirsch E, Liu F, Daumke O, Kudryashev M, Haucke V

Nature structural & molecular biology 29 (2022) 218-228

PMID: 35256802

DOI: 10.1038/s41594-022-00730-w

Related EMDB entry:

EMD-12191

Deposited:

2020-06-17

Released:

2022-03-28

Last modified:

2022-03-28

Imageset size:

---

Imageset DOI:

10.6019/EMPIAR-10665

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

Movies in this entry were compressed to TIFF by EMPIAR Japan to save space and bandwidth.
The compression is lossless in that all pixel intensities remain exactly the same after decompression.
However, some metadata in the MRC header were lost.
The original files before compression are available from EMPIAR EBI.

Original size:   10,173,743,848 bytes
Compressed size:  5,477,067,974 bytes

Contains:

micrographs - multiframe

Image Sets

1. -30 degree tilted PI3KC2a data set raw movies

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

6568

Image size:

(5760, 4092)

Pixel type:

SIGNED BYTE

Pixel spacing:

(0.837, 0.837)

Details:

The folder contains raw movies collected with stage -30 degree tilted, total dose is 60 electrons per squared Angstrom. Each movie has 50 frames.

Files:

Archives and downloads the selected files into an uncompressed zip file.

Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

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Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. untilted PI3KC2a data set raw movies

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

3865

Image size:

(5760, 4092)

Pixel type:

SIGNED BYTE

Pixel spacing:

(0.837, 0.837)

Details:

The folder contains raw movies collected with stage untilted, total dose is about 60 electrons per squared Angstrom. Each movie has 50 frames.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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ICECREAM: high-fidelity equivariant cryo-electron tomography.

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CryoSift: an accessible and automated CNN-driven tool for cryo-EM 2D class selection.

Schäfer JH, Calza A, Hom K, Damodar P, Peng R, Bogdanović N, Lander GC, Stagg SM, Cianfrocco MA. (2025)