EMPIAR-10616 - Cryo-EM dataset of human pre-Bact spliceosome [multiple data sets in MRC format]

EMPIAR-10616

Cryo-EM dataset of human pre-Bact spliceosome [multiple data sets in MRC format]

Publication:

Mechanism of protein-guided folding of the active site U2/U6 RNA during spliceosome activation

Townsend C, Leelaram MN, Agafonov DE, Dybkov O, Will CL, Bertram K, Urlaub H, Kastner B, Stark H, Lührmann R

Science (New York, N.Y.) 370 (2020)

PMID: 33243851

DOI: 10.1126/science.abc3753

Related EMDB entry:

EMD-11697 EMD-11695 EMD-11694 EMD-11696 EMD-11693

Deposited:

2020-09-07

Released:

2021-01-29

Last modified:

2021-11-02

Imageset size:

584.51 GB

Imageset DOI:

10.6019/EMPIAR-10616

Experimental metadata:

Download xml json

Contains:

micrographs - single frame

Image Sets

1. Motion-corrected micrographs (without dose-weighting) of human pre-Bact spliceosome

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

4676

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.16, 1.16)

Details:

Motion-corrected micrographs (without dose-weighting). Recorded with 45 electrons per squared angstrom over 20 frames at a pixel size of 1.16A and a fluence of 2.25e/A^2/frame.

Files:

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2. Motion-corrected micrographs (with dose-weighting) of human pre-Bact spliceosome

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

4676

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.16, 1.16)

Details:

Motion-corrected micrographs (with dose-weighting). Recorded with 45 electrons per squared angstrom over 20 frames at a pixel size of 1.16A and a fluence of 2.25e/A^2/frame.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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