EMPIAR-10616 - Cryo-EM dataset of human pre-Bact spliceosome [multiple data sets in MRC format]

EMPIAR-10616

Cryo-EM dataset of human pre-Bact spliceosome [multiple data sets in MRC format]

Publication:

Mechanism of protein-guided folding of the active site U2/U6 RNA during spliceosome activation

Townsend C, Leelaram MN, Agafonov DE, Dybkov O, Will CL, Bertram K, Urlaub H, Kastner B, Stark H, Lührmann R

Science (New York, N.Y.) 370 (2020)

PMID: 33243851

DOI: 10.1126/science.abc3753

Related EMDB entry:

EMD-11697 EMD-11695 EMD-11694 EMD-11696 EMD-11693

Deposited:

2020-09-07

Released:

2021-01-29

Last modified:

2021-11-02

Imageset size:

584.51 GB

Imageset DOI:

10.6019/EMPIAR-10616

Experimental metadata:

Download xml json

Contains:

micrographs - single frame

Image Sets

1. Motion-corrected micrographs (without dose-weighting) of human pre-Bact spliceosome

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

4676

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.16, 1.16)

Details:

Motion-corrected micrographs (without dose-weighting). Recorded with 45 electrons per squared angstrom over 20 frames at a pixel size of 1.16A and a fluence of 2.25e/A^2/frame.

Files:

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2. Motion-corrected micrographs (with dose-weighting) of human pre-Bact spliceosome

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

4676

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.16, 1.16)

Details:

Motion-corrected micrographs (with dose-weighting). Recorded with 45 electrons per squared angstrom over 20 frames at a pixel size of 1.16A and a fluence of 2.25e/A^2/frame.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

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Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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