EMPIAR-10613 - DNA origami signposts for identifying proteins on cell membranes by electron cryotomography [2 tilt series in MRC format]

EMPIAR-10613

DNA origami signposts for identifying proteins on cell membranes by electron cryotomography [2 tilt series in MRC format]

Publication:

DNA origami signposts for identifying proteins on cell membranes by electron cryotomography

Silvester E, Vollmer B, Pražák V, Vasishtan D, Machala EA, Whittle C, Black S, Bath J, Turberfield AJ, Grünewald K, Baker LA

Cell 184 (2021) 1110-1121.e16

PMID: 33606980

DOI: 10.1016/j.cell.2021.01.033

Related EMDB entry:

EMD-12188

Deposited:

2020-12-16

Released:

2021-04-14

Last modified:

2021-06-30

Imageset size:

40.64 MB

Imageset DOI:

10.6019/EMPIAR-10613

Experimental metadata:

Download xml json

Contains:

tilt series

Image Sets

1. Tomograms of sfGFP-glycoprotein B vesicles with DNA origami SPOTs

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(None, None)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(16.32, 16.32)

Details:

Tilt series were phase flipped and binned 2x with IMOD prior to reconstruction. Tomograms were then binned a further 3x and bandpass filtered between 2000 and 300 Å with Bsoft.

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

EMICSS: added-value annotations for EMDB entries.

Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)

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