EMPIAR-10612 - High-resolution SARS-CoV-2 ORF3a dimer in an MSP1E3D1 lipid nanodisc [multiple data sets in EER format]

EMPIAR-10612

High-resolution SARS-CoV-2 ORF3a dimer in an MSP1E3D1 lipid nanodisc [multiple data sets in EER format]

Publication:

Cryo-EM structure of the SARS-CoV-2 3a ion channel in lipid nanodiscs

Kern DM, Sorum B, Hoel CM, Sridharan S, Remis JP, Toso DB, Brohawn SG

bioRxiv (2020)

DOI: 10.1101/2020.06.17.156554

Related EMDB entry:

EMD-22898

Deposited:

2020-11-28

Released:

2021-02-05

Last modified:

2023-02-20

Imageset size:

3.16 TB

Imageset DOI:

10.6019/EMPIAR-10612

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned movies in EER format of SARS-CoV-2 3a in MSP1E3D1 lipid nanodiscs - 1379 frames

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

789

Image size:

(4096, 4096)

Pixel type:

BIT

Pixel spacing:

(0.3635, 0.3635)

Details:

EER-format movies are in folders titled by total frame number. We recommend merging 30 frames for one dose fraction which gives 45 frame movies. The dose per frame is 0.0345 e/A2 (or 1.035e/A2 per suggested fraction). The gain reference image is in the movies folder. The super-resolution pixel size is 0.3635 A for a physical pixel size of 0.727 A. A cryosparc non-uniform refinement folder (containing polished particles) is also included.

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2. Unaligned movies in EER format of SARS-CoV-2 3a in MSP1E3D1 lipid nanodiscs - 1449 frames

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

4809

Image size:

(4096, 4096)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.3635, 0.3635)

Details:

EER-format movies are in folders titled by total frame number. We recommend merging 30 frames for one dose fraction which gives 48 frame movies. The dose per frame is 0.0345 e/A2 (or 1.035e/A2 per suggested fraction). The gain reference image is in the movies folder. The super-resolution pixel size is 0.3635 A for a physical pixel size of 0.727 A. A cryosparc non-uniform refinement folder (containing polished particles) is also included.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

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