EMPIAR-10604 - Single particle cryo-EM dataset of Mus musculus mitochondrial complex I in the deactive state [1768 multi-frame micrographs composed of 30 frames each in MRCS format]

EMPIAR-10604

Single particle cryo-EM dataset of Mus musculus mitochondrial complex I in the deactive state [1768 multi-frame micrographs composed of 30 frames each in MRCS format]

Publication:

Structural basis for a complex I mutation that blocks pathological ROS production

Yin Z, Burger N, Kula-Alwar D, Aksentijević D, Bridges HR, Prag H, Grba D, Viscomi C, James AJ, Mottahedin A, Kreig T, Murphy MP, Hirst J

Nature communications 12 (2021)

PMID: 33514727

DOI: 10.1038/s41467-021-20942-w

Related EMDB entry:

EMD-11810 EMD-4356

Deposited:

2020-12-07

Released:

2021-02-12

Last modified:

2021-02-12

Imageset size:

3.02 TB

Imageset DOI:

10.6019/EMPIAR-10604

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Single particle cryo-EM dataset of Mus musculus mitochondrial complex I in the deactive state

Category:

micrographs - multiframe

Image format:

MRCS

No. of images or tilt series:

1768

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.35, 1.35)

Details:

Micrographs were imaged using a Gatan K2 summit camera in counting mode and GIF Quantum energy filter (20 eV slit width) on an FEI 300 keV Titan Krios microscope with a 100 μm objective aperture. 15 s exposures captured in 30 frames and a total dose of 50 electrons Å-2

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

EMICSS: added-value annotations for EMDB entries.

Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)

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