EMPIAR-10590 - Single-particle Cryo-EM of the endogenous human BAF complex alone [4865 micrographs in MRC format]

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EMPIAR-10590

Single-particle Cryo-EM of the endogenous human BAF complex alone [4865 micrographs in MRC format]

Publication:

A Structural Model of the Endogenous Human BAF Complex Informs Disease Mechanisms

Mashtalir N, Suzuki H, Farrell DP, Sankar A, Luo J, Filipovski M, D'Avino AR, St Pierre R, Valencia AM, Onikubo T, Roeder RG, Han Y, He Y, Ranish JA, DiMaio F, Walz T, Kadoch C

Cell 183 (2020) 802-817

PMID: 33053319

DOI: 10.1016/j.cell.2020.09.051

Related EMDB entry:

EMD-22476

Deposited:

2020-10-15

Released:

2020-12-22

Last modified:

2020-12-22

Imageset size:

258.07 GB

Imageset DOI:

10.6019/EMPIAR-10590

Experimental metadata:

Download xml json

Contains:

micrographs - single frame

Image Sets

1. Motion-corrected micrographs of the endogenous human BAF complex on thin-layer carbon grids

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

4865

Image size:

(3710, 3838)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.5, 1.5)

Details:

Motion-corrected averages of movies collected at an accumulated dose of 66.7 e-/A^2 across 50 frames over a 15 s exposure, recorded on Talos Arctica (FEI) with a K2 Summit direct detector (Gatan) in super-resolution counting mode. Motion correction was performed using MotionCor2 with dose weighting, 2x binning, and 5x5 patches.

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