EMPIAR-10550 - Structure of the class D GPCR Ste2 dimer coupled to two G proteins [multiple data sets in MRC and TIFF formats]

EMPIAR-10550

Structure of the class D GPCR Ste2 dimer coupled to two G proteins [multiple data sets in MRC and TIFF formats]

Publication:

Structure of the class D GPCR Ste2 dimer coupled to two G proteins

Velazhahan V, Ma N, Pándy-Szekeres G, Kooistra AJ, Lee Y, Gloriam DE, Vaidehi N, Tate CG

Nature 589 (2020) 148-153

PMID: 33268889

DOI: 10.1038/s41586-020-2994-1

Related EMDB entry:

EMD-11720

Deposited:

2020-09-14

Released:

2020-12-04

Last modified:

2021-02-12

Imageset size:

1.28 TB

Imageset DOI:

10.6019/EMPIAR-10550

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

--- file not found ---

Contains:

micrographs - multiframe

Image Sets

1. LMB Krios1 Movies

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

996

Image size:

(3838, 3710)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.145, 1.145)

Details:

contains unaligned movies corresponding to the data collection "LMB Krios1". The real pixel size is 1.145 Å/pix, and the dose rate is 1.013 e/Å^2/frame. Please use data/LMB_Krios1_Movies/Micrographs/GainReference/FoilHole_16392868_Data_16385023_16385025_20191017_011206-gain-ref.MRC as gain reference without any rotation or flipping.

Files:

Archives and downloads the selected files into an uncompressed zip file.

Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. LMB Krios2 Movies

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2027

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.1, 1.1)

Details:

contains unaligned movies corresponding to the data collection "LMB Krios2". The real pixel size is 1.1 Å/pix, and the dose rate is 1.015 e/Å^2/frame. Please use data/LMB_Krios2_Movies/Micrographs/h1_s1/CountRef_krios2_24102019_h1_s1_i0001_movie_Oct24_17.44.14.mrc as gain reference without any rotation or flipping. The defects file can be found at data/LMB_Krios2_Movies/Micrographs/h1_s1/defects_krios2_24102019_h1_s1_i0001_movie_Oct24_17.44.14.txt. The "_hn_sn_" ,where n=1-9, portion of the file name refers to the hole and shot position within 3x3 image shift acquisition matrix (two non-overlapping shots per hole was acquired).

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. eBic Krios1 Movies

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

4637

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.047, 1.047)

Details:

contains unaligned movies corresponding to the data collection "eBic Krios1". The real pixel size is 1.047 Å/pix, and the dose rate is 1.003 e/Å^2/frame. Please use data/eBIC_Krios1_Movies/Gain_reference/gain.mrc as gain reference without any rotation or flipping.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Quick Links

Browse EMPIAR

Sample Preparation Widget

Volume Browser

Tomo Viewer Prototype

Claim entries to your ORCiD

Talks and Tutorials

EMPIAR Quick tour

Publications

Re-use study

Statistics

EMPIAR in the news

COVID-19 Data Portal

EMDB

BioImage Archive

PDBe

EMNavigator@PDBj

PDBj

Citations

prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

He L, Bartesaghi A. (2026)

CryoSIP: unleashing protein high-resolution Cryo-EM via semantic-instance collaborative picking.

Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)

Unbend, correction of local beam-induced sample motion in cryo-EM images using a 3D spline model.

Kong L, Zottig X, Elferich J, Grigorieff N. (2026)

Chart builder: an interactive tool for user driven data visualization in the electron microscopy data bank.

Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

Dual site targeting of the bacterial 70S ribosome by tetracyclines.

Devarkar SC, Lomakin IB, Wang J, Grada A, Bunick CG. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements.

Jones HN, Deshmukh A, Pande K. (2026)

CryoGS: High-Quality Cryo-EM Homogeneous Reconstruction by Gaussian Splatting

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)

Point cloud deformation modeling for particle selection following cryo-EM 2D classification.

Wang X, Mo Z, Li F, Zhang F, Wan X. (2026)

ICECREAM: high-fidelity equivariant cryo-electron tomography.

Kishore V, Debarnot V, Righetto RD, Engel BD, Dokmanić I. (2026)