EMPIAR-10550 - Structure of the class D GPCR Ste2 dimer coupled to two G proteins [multiple data sets in MRC and TIFF formats]

EMPIAR-10550

Structure of the class D GPCR Ste2 dimer coupled to two G proteins [multiple data sets in MRC and TIFF formats]

Publication:

Structure of the class D GPCR Ste2 dimer coupled to two G proteins

Velazhahan V, Ma N, Pándy-Szekeres G, Kooistra AJ, Lee Y, Gloriam DE, Vaidehi N, Tate CG

Nature 589 (2020) 148-153

PMID: 33268889

DOI: 10.1038/s41586-020-2994-1

Related EMDB entry:

EMD-11720

Deposited:

2020-09-14

Released:

2020-12-04

Last modified:

2021-02-12

Imageset size:

1.28 TB

Imageset DOI:

10.6019/EMPIAR-10550

Experimental metadata:

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Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

MRC movies (FoilHole*.mrc) in this entry were compressed by bzip2 (pbzip2)
by EMPIAR Japan to save space and bandwidth.

Some programs (e.g. RELION >= 4.0.1 and CryoSPARC) can process compressed movies without explicit
decompression. For other programs, movies have to be decompressed beforehand. In this case,
you might want to convert decompressed MRC movies into TIFF to save your local storage.

Original size:   6,466,617,150 kilobytes
Compressed size:   993,373,678 kilobytes

Contains:

micrographs - multiframe

Image Sets

1. LMB Krios1 Movies

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

996

Image size:

(3838, 3710)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.145, 1.145)

Details:

contains unaligned movies corresponding to the data collection "LMB Krios1". The real pixel size is 1.145 Å/pix, and the dose rate is 1.013 e/Å^2/frame. Please use data/LMB_Krios1_Movies/Micrographs/GainReference/FoilHole_16392868_Data_16385023_16385025_20191017_011206-gain-ref.MRC as gain reference without any rotation or flipping.

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2. LMB Krios2 Movies

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2027

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.1, 1.1)

Details:

contains unaligned movies corresponding to the data collection "LMB Krios2". The real pixel size is 1.1 Å/pix, and the dose rate is 1.015 e/Å^2/frame. Please use data/LMB_Krios2_Movies/Micrographs/h1_s1/CountRef_krios2_24102019_h1_s1_i0001_movie_Oct24_17.44.14.mrc as gain reference without any rotation or flipping. The defects file can be found at data/LMB_Krios2_Movies/Micrographs/h1_s1/defects_krios2_24102019_h1_s1_i0001_movie_Oct24_17.44.14.txt. The "_hn_sn_" ,where n=1-9, portion of the file name refers to the hole and shot position within 3x3 image shift acquisition matrix (two non-overlapping shots per hole was acquired).

Files:

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3. eBic Krios1 Movies

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

4637

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.047, 1.047)

Details:

contains unaligned movies corresponding to the data collection "eBic Krios1". The real pixel size is 1.047 Å/pix, and the dose rate is 1.003 e/Å^2/frame. Please use data/eBIC_Krios1_Movies/Gain_reference/gain.mrc as gain reference without any rotation or flipping.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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