EMPIAR-10544
Cryo-EM structure of a dimeric B-Raf:14-3-3 complex reveals asymmetry in the active sites of B-Raf kinases [multiple data sets in MRCS and MRC formats]
Publication:

Cryo-EM structure of a dimeric B-Raf:14-3-3 complex reveals asymmetry in the active sites of B-Raf kinases

Kondo Y, Ognjenović J, Banerjee S, Karandur D, Merk A, Kulhanek K, Wong K, Roose JP, Subramaniam S, Kuriyan J

Science (New York, N.Y.) 366 (2019) 109-115

PMID: 31604311

Related EMDB entry:
Deposited:
2020-10-15
Released:
2022-06-22
Last modified:
2022-06-22
Imageset size:
934.30 GB
Imageset DOI:
Experimental metadata:
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Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

Movies (Dataset?/20*.mrc) in this entry were compressed to TIFF by EMPIAR Japan to save space and bandwidth.
The compression is lossless in that all pixel intensities remain exactly the same after decompression.
However, some metadata in the MRC header were lost.
The original files before compression are available from EMPIAR EBI.

Original size:   3,228,248,644 kilobytes
Compressed size:   979,239,148 kilobytes
Contains:
  • micrographs - multiframe
1. Unaligned multi-frame micrographs of B-Raf:14-3-3 complex
Category:
micrographs - multiframe
Image format:
MRCS
No. of images or tilt series:
1539
Image size:
(7420, 7676)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.532, 0.532)
Details:
The micrographs are the unaligned, unnormalized super-resolution movie frames (40 frames per micrograph). Total accumulated dose is 50 electrons/Å^2 over a 12 seconds exposure. GainRef.mrc can be used with 270 degrees rotation.
Files:
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2. Unaligned multi-frame micrographs of B-Raf:14-3-3 complex
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
1451
Image size:
(7420, 7676)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.532, 0.532)
Details:
The micrographs are the unaligned, unnormalized super-resolution movie frames (40 frames per micrograph). Total accumulated dose is 50 electrons/Å^2 over a 12 seconds exposure. GainRef.mrc requires no rotation.
Files:
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