EMPIAR-10536 - Single particle cryo-EM dataset for MlaFEDB from E. coli in nanodisc [multiple data sets in MRC and MRCS formats]

EMPIAR-10536

Single particle cryo-EM dataset for MlaFEDB from E. coli in nanodisc [multiple data sets in MRC and MRCS formats]

Publication:

Structure of bacterial phospholipid transporter MlaFEDB with substrate bound

Coudray N, Isom GL, MacRae M, Saiduddin MN, Bhabha G, Ekiert DC

eLife 9 (2020)

PMID: 33236984

DOI: 10.7554/elife.62518

Related EMDB entry:

EMD-22116

Deposited:

2020-06-10

Released:

2020-12-09

Last modified:

2021-01-29

Imageset size:

996.26 GB

Imageset DOI:

10.6019/EMPIAR-10536

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

Movies (*frames.mrc) in this entry were compressed to TIFF by EMPIAR Japan to save space and bandwidth.
The compression is lossless in that all pixel intensities remain exactly the same after decompression.
However, some metadata in the MRC header were lost.
The original files before compression are available from EMPIAR EBI.

The original entry contained two temporary files, which were removed.
- data/movies/18aug01e_nc0030_00022gr_00026sq_v02_00015hln_00004esn.frames.mrc.partial
- data/movies/18aug01e_nc0030_00022gr_00026sq_v02_00015hln_00004esn.frames.mrc.aspera-ckpt

Original size:   5,359,644,379 kilobytes
Compressed size:   869,949,652 kilobytes

Contains:

micrographs - multiframe
picked particles - single frame - processed

Image Sets

1. Unaligned multi-frame micrographs of MlaFEDB complex

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

3212

Image size:

(7420, 7676)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.416, 0.416)

Details:

Unaligned super-resolution (K2 camera, Titan Krios) multi-frame micrographs (total electron dose: 71 e-/A2; frame rate: 200 ms)

Files:

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2. Polished particles (using Relion 3.1)

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

3064

Image size:

(500, 500)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.832, 0.832)

Details:

Picked particles after polishing in Relion 3.1.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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Browse All Files

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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