Single particle cryoEM of Streptococcus thermophilus SHP pheromone receptor Rgg3 in complex with SHP3 [1464 multi-frame micrographs composed of 40 frames each in MRC format]
Publication:
Structure-function studies of Rgg binding to pheromones and target promoters reveal a model of transcription factor interplay
The dataset contains unaligned gain-corrected micrograph movies in mrc format. Cryo-EM data were collected at the Rutgers New Jersey CryoEM/CryoET Core Facility using a 200 kV Talos Arctica (FEI/ThermoFisher) electron microscope equipped with a K2 Summit direct electron detector (Gatan) and a GIF Quantum energy filter (Gatan) with slit width of 20 eV. Data were collected automatically in counting mode using EPU (FEI/ThermoFisher), a nominal magnification of 130,000X, a calibrated pixel size of 1.038 Å per pixel, and a dose rate of 6.347 electrons/pixel/s. Movies were recorded at 200 ms/frame for 8 s (40 frames total), resulting in a total radiation dose of 47.13 electrons/Å2. Defocus range was 0.5 to 3.5 μm. A total of 1,464 micrographs were recorded from two grids over two days. Micrographs were gain-normalized and defect corrected.
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Archives and downloads the selected files into an uncompressed zip file.
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Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.
We recommend using rsync, aspera, globus, etc.
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