EMPIAR-10535 - Single particle cryoEM of Streptococcus thermophilus SHP pheromone receptor Rgg3 in complex with SHP3 [1464 multi-frame micrographs composed of 40 frames each in MRC format]

EMPIAR-10535

Single particle cryoEM of Streptococcus thermophilus SHP pheromone receptor Rgg3 in complex with SHP3 [1464 multi-frame micrographs composed of 40 frames each in MRC format]

Publication:

Structure-function studies of Rgg binding to pheromones and target promoters reveal a model of transcription factor interplay

Capodagli GC, Tylor KM, Kaelber JT, Petrou VI, Federle MJ, Neiditch MB

Proceedings of the National Academy of Sciences of the United States of America 117 (2020) 24494-24502

PMID: 32907945

DOI: 10.1073/pnas.2008427117

Related EMDB entry:

EMD-22341

Deposited:

2020-07-22

Released:

2020-11-13

Last modified:

2020-11-13

Imageset size:

3.03 TB

Imageset DOI:

10.6019/EMPIAR-10535

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of Rgg3-SHP3 complex

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

1464

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.038, 1.038)

Details:

The dataset contains unaligned gain-corrected micrograph movies in mrc format. Cryo-EM data were collected at the Rutgers New Jersey CryoEM/CryoET Core Facility using a 200 kV Talos Arctica (FEI/ThermoFisher) electron microscope equipped with a K2 Summit direct electron detector (Gatan) and a GIF Quantum energy filter (Gatan) with slit width of 20 eV. Data were collected automatically in counting mode using EPU (FEI/ThermoFisher), a nominal magnification of 130,000X, a calibrated pixel size of 1.038 Å per pixel, and a dose rate of 6.347 electrons/pixel/s. Movies were recorded at 200 ms/frame for 8 s (40 frames total), resulting in a total radiation dose of 47.13 electrons/Å2. Defocus range was 0.5 to 3.5 μm. A total of 1,464 micrographs were recorded from two grids over two days. Micrographs were gain-normalized and defect corrected.

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