EMPIAR-10518 - Cryo-EM structure of immunodominant protein P1 from human pathogen Mycoplasma pneumoniae on graphene oxide grid [multiple data sets in TIFF format]

EMPIAR-10518

Cryo-EM structure of immunodominant protein P1 from human pathogen Mycoplasma pneumoniae on graphene oxide grid [multiple data sets in TIFF format]

Publication:

Immunodominant proteins P1 and P40/P90 from human pathogen Mycoplasma pneumoniae

Vizarraga D, Kawamoto A, Matsumoto U, Illanes R, Pérez-Luque R, Martín J, Mazzolini R, Bierge P, Pich OQ, Espasa M, Sanfeliu I, Esperalba J, Fernández-Huerta M, Scheffer MP, Pinyol J, Frangakis AS, Lluch-Senar M, Mori S, Shibayama K, Kenri T, Kato T, Namba K, Fita I, Miyata M, Aparicio D

Nature communications 11 (2020)

PMID: 33057023

DOI: 10.1038/s41467-020-18777-y

Related EMDB entry:

EMD-30233

Deposited:

2020-09-01

Released:

2020-11-11

Last modified:

2020-11-11

Imageset size:

8.04 TB

Imageset DOI:

10.6019/EMPIAR-10518

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned 136-frame movies of human pathogen Mycoplasma pneumoniae P1

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

512

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(0.88, 0.88)

Details:

human pathogen Mycoplasma pneumoniae P1(particles have preferred orientations). Cryo-EM imaging was obtained using a Titan Krios operating at 300 kV acceleration voltage and equipped with a Cs corrector and a Falcon III direct electron detector. A total of 512 movies were obtained in the electron counting mode with a physical pixel size of 0.88 Å/pixel and total dose of 60 e/Å2 with 63.2 s exposure time. The data were automatically collected using EPU software with a defocus range of -1.25 to -2.75 µm and were fractionated into 136 movie frames.

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2. Unaligned 78-frame movies of human pathogen Mycoplasma pneumoniae P1

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

5469

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(0.69, 0.69)

Details:

human pathogen Mycoplasma pneumoniae P1 on graphene oxide grid. Cryo-EM imaging was obtained using a Titan Krios operating at 300 kV acceleration voltage and equipped with a Cs corrector and a Falcon III direct electron detector. A total of 5469 movies were obtained in the electron counting mode with a physical pixel size of 0.69 Å/pixel and total dose of 60 e/Å2 with 36.33 s exposure time. The data were automatically collected using EPU software with a defocus range of -0.75 to -2.75 µm and were fractionated into 78 movie frames.

Files:

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Browse All Files

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Download a list of selected files.

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