EMPIAR-10452
Cryo Electron Tomograms of Membrane Fractions of Rabbit Skeletal Muscle for Structural Determination of RyR1 in SR Vesicles at subnanometer resolution [48 tilt series in MRC format]
Publication:

Subnanometer-resolution structure determination in situ by hybrid subtomogram averaging - single particle cryo-EM

Sanchez RM, Zhang Y, Chen W, Dietrich L, Kudryashev M

Nature communications 11 (2020)

PMID: 32709843

Related EMDB entry:
Deposited:
2020-06-22
Released:
2020-08-18
Last modified:
2020-08-18
Imageset size:
435.81 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • tilt series
1. 48 tilt series (frames aligned my motioncorr2) in .st stacks (mrc format for imod) including .tlt, .xf and .defocus files to generate reconstructions
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
48
Image size:
(3710, 3838)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.7, 1.7)
Details:
The alignment parameters .tlt and .xf files could be used to perform 3D reconstruction using Imod; the locations of the particles and the alignment parameters are located in the .tbl file (Dynamo-style). Column 23 in the table indicates the separation into independent half-sets.
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