EMPIAR-10452 - Cryo Electron Tomograms of Membrane Fractions of Rabbit Skeletal Muscle for Structural Determination of RyR1 in SR Vesicles at subnanometer resolution [48 tilt series in MRC format]

EMPIAR-10452

Cryo Electron Tomograms of Membrane Fractions of Rabbit Skeletal Muscle for Structural Determination of RyR1 in SR Vesicles at subnanometer resolution [48 tilt series in MRC format]

Publication:

Subnanometer-resolution structure determination in situ by hybrid subtomogram averaging - single particle cryo-EM

Sanchez RM, Zhang Y, Chen W, Dietrich L, Kudryashev M

Nature communications 11 (2020)

PMID: 32709843

DOI: 10.1038/s41467-020-17466-0

Related EMDB entry:

EMD-10840

Deposited:

2020-06-22

Released:

2020-08-18

Last modified:

2020-08-18

Imageset size:

435.81 GB

Imageset DOI:

10.6019/EMPIAR-10452

Experimental metadata:

Download xml json

Contains:

tilt series

Image Sets

1. 48 tilt series (frames aligned my motioncorr2) in .st stacks (mrc format for imod) including .tlt, .xf and .defocus files to generate reconstructions

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(3710, 3838)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.7, 1.7)

Details:

The alignment parameters .tlt and .xf files could be used to perform 3D reconstruction using Imod; the locations of the particles and the alignment parameters are located in the .tbl file (Dynamo-style). Column 23 in the table indicates the separation into independent half-sets.

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