EMPIAR logo

Electron Microscopy Public Image Archive

EMPIAR-10434
TEM tomograms of Drosophila tracheal terminal cells during subcellular tube formation [multiple data sets in TIFF and MRC formats]
Publication:

Transcytosis via the late endocytic pathway as a cell morphogenetic mechanism

Mathew R, Rios-Barrera LD, Machado P, Schwab Y, Leptin M

The EMBO journal (2020)

PMID: 32657472

Related EMDB entry:
Deposited:
2020-06-10
Released:
2020-08-12
Last modified:
2020-08-12
Imageset size:
366.68 GB
Imageset DOI:
10.6019/EMPIAR-10434
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
Image Sets
1. Aligned EM tomographs covering an embryonic tracheal terminal cell, overview image
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(None, None)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(25.49, 25.49)
Details:
Tilt series were performed in 1 degree increments from 60 to -60 degrees with 2.549 nm pixel size on a FEI Tecnai F30 electron microscope with a Gatan OneView camera. The serial tomograms were reconstructed and aligned using the IMOD package.
Files:
Loading...
2. Aligned EM tomographs covering an embryonic tracheal terminal cell, overview image
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(None, None)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(25.49, 25.49)
Details:
Tilt series were performed in 1 degree increments from 60 to -60 degrees with 2.549 nm pixel size on a FEI Tecnai F30 electron microscope with a Gatan OneView camera. The serial tomograms were reconstructed and aligned using the IMOD package.
Files:
Loading...
3. Aligned EM tomographs covering an embryonic tracheal terminal cell after 1 hour of dynamin inactivation
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(None, None)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(25.49, 25.49)
Details:
Tilt series were performed in 1 degree increments from 60 to -60 degrees with 2.549 nm pixel size on a FEI Tecnai F30 electron microscope with a Gatan OneView camera. The serial tomograms were reconstructed and aligned using the IMOD package.
Files:
Loading...
4. Aligned EM tomographs covering an embryonic tracheal terminal cell after 1 hour of dynamin inactivation
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1
Image size:
(None, None)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(25.49, 25.49)
Details:
Tilt series were performed in 1 degree increments from 60 to -60 degrees with 2.549 nm pixel size on a FEI Tecnai F30 electron microscope with a Gatan OneView camera. The serial tomograms were reconstructed and aligned using the IMOD package.
Files:
Loading...
5. Tomographs covering subpositions within an embryonic tracheal terminal cell
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
45
Image size:
(4096, 4096)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(7.8, 7.8)
Details:
Data is organized by grids (g*) where the physical sections (s*) were present; hm* refers to the number of high-magnification images obtained from a given section. Tomograms are .mrc files, saved as .rec files. 3D reconstruction models are saved in the same folder as .mod and are also .mrc files generated using IMOD.
Files:
Loading...
6. Tomographs covering subpositions within an embryonic tracheal terminal cell
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
36
Image size:
(4096, 4096)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(7.8, 7.8)
Details:
Data is organized by grids (g*) where the physical sections (s*) were present; hm* refers to the number of high-magnification images obtained from a given section. Tomograms are .mrc files, saved as .rec files. 3D reconstruction models are saved in the same folder as .mod and are also .mrc files generated using IMOD.
Files:
Loading...
Browse All Files
Quick Links
Talks and Tutorials
EMPIAR Quick tour
Statistics
Publications
Re-use case study
EMPIAR in the news
Claim entries to your ORCiD
COVID-19 Data Portal
Contact us
EMDB
PDBe
BioImage Archive
EMNavigator@PDBj
PDBj
RCSB@PDBj
EMDataResoruce@RCSB,EMDB,NCMI
Citations
Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.
Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)
Outer membrane protein assembly mediated by BAM-SurA complexes.
Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)
Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.
Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)
Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.
Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)
The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.
Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)
See all citations
Protein Data Bank Japan News
[2024-11-20]
224 new PDB entries have been released on 2024-11-20.
[2024-11-01]
A new portal integrating genome variants and protein structures has released
[2024-10-30]
[wwPDB] Deprecation of FTP File Download Protocol on November 1, 2024
[2024-10-25]
PDBj luncheon seminar at APBJC 2024
[2024-10-17]
[wwPDB] The Nobel Prize in Chemistry 2024
[2024-10-16]
[wwPDB] wwPDB Data Release Policy on Public Preprint Archives
[2024-10-09]
[wwPDB] Annotation of Protein Modifications in the PDB
[2024-10-03]
[wwPDB] Access IHM structures at wwPDB DOI Landing Pages
[2024-09-18]
[wwPDB] Deprecation of FTP File Download Protocol on November 1, 2024
[2024-09-12]
[wwPDB] Paper Published on CryoEM Archiving and Validation Recommendations
See all news