3,168 movies were acquired from two grids of apoferritin prepared with BIU. The pixel size was calibrated at 0.816 Å/pixel by fitting a crystal structure of human ferritin L chain (PDB ID: 2FFX) into a preliminary 3D map. Movies were collected for 9 sec with 29 exposure fractions, a camera exposure rate of ~3 e-/pix/sec, and total specimen exposure of ~40 e-/Å^2. Note: Due to software issues, the last frames in these raw movies have errors. Please exclude the last frame (frame 30) when doing any alignments.
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Exposure fractions were aligned with MotionCor2 within Relion, using 7x7 patches and a B factor of 500 pixel^2 for global alignment and 150 pixel^2 for local alignment. There is a micrographs_ctf.star file with CTF estimation from CTFFind4. Note: Due to software issues, the last frames in these raw movies have errors. Please exclude the last frame (frame 30) when doing any alignments.
Files:
Available download options:
Archives and downloads the selected files into an uncompressed zip file.
Depending on the number and size of files to be downloaded, this can take an enormous amount of time.
Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.
We recommend using rsync, aspera, globus, etc.
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Final polished particle stack with Euler angles and shifts. Higher order CTF aberration corrections were done within CryoSPARC 2 but the resulting parameters were not able to be exported to .star format here. When using this particle stack, it is recommended to do higher order CTF aberration corrections again in CryoSPARC/Relion to obtain the best resolution.
Files:
Available download options:
Archives and downloads the selected files into an uncompressed zip file.
Depending on the number and size of files to be downloaded, this can take an enormous amount of time.
Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.
We recommend using rsync, aspera, globus, etc.
Download a list of selected files.
It is possible to download files by specifying the file list with rsync command, etc.
Archives and downloads the selected files into an uncompressed zip file.
Depending on the number and size of files to be downloaded, this can take an enormous amount of time.
Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.
We recommend using rsync, aspera, globus, etc.
Download a list of selected files.
It is possible to download files by specifying the file list with rsync command, etc.
