EMPIAR-10399
Single-Particle Cryo-EM of Arabinofuranosyltransferase AftD from Mycobacteria, Wild-type [multiple data sets in MRCS and MRC formats]
Publication:

Cryo-EM Structures and Regulation of Arabinofuranosyltransferase AftD from Mycobacteria

Tan YZ, Zhang L, Rodrigues J, Zheng RB, Giacometti SI, Rosário AL, Kloss B, Dandey VP, Wei H, Brunton R, Raczkowski AM, Athayde D, Catalão MJ, Pimentel M, Clarke OB, Lowary TL, Archer M, Niederweis M, Potter CS, Carragher B, Mancia F

Molecular cell (2020)

PMID: 32386575

Related EMDB entry:
Deposited:
2020-03-27
Released:
2020-05-26
Last modified:
2020-04-21
Imageset size:
2.70 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • micrographs - single frame
  • picked particles - single frame - processed
1. Unaligned and compressed multi-frame movies
Category:
micrographs - multiframe
Image format:
MRCS
No. of images or tilt series:
7354
Image size:
(3838, 3710)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(1.0605, 1.0605)
Details:
Data collection was performed using a dose of ~95.72 e-/Å2 across 90 frames (150 msec per frame) at a dose rate of ~8.2 e-/pix/sec, using a set defocus range of -1.0 μm to -2.0 μm. Raw frames were compressed using pbzip2. Gain reference image to use are in each of the four folders under references/*_3838x3710_norm_0_mod.mrc.
Files:
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2. Aligned and dose-weighted micrographs
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
7274
Image size:
(3838, 3710)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.0605, 1.0605)
Details:
Movie frames were aligned using MotionCor2 with 5 by 5 patches and B-factor of 100 through the Appion software package.
Files:
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3. Final Particle Stacks with Refined Euler Angles and Shifts (Overall and Focused Refined on CBM3)
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
2
Image size:
(288, 288)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.0605, 1.0605)
Details:
Final particle stacks, with Relion .star file containing the refined Euler angles and shifts. Final_AftD-WT.star has the refined Euler angles and shifts for the entire dataset, while FocussedClassified_CBM3_AftD-WT.star has the refined Euler angles and shifts for just the subset of particles after CBM3 focused classification.
Files:
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