EMPIAR-10384
Cryo-EM structure of Lumazine Synthase [1198 multi-frame micrographs composed of 41 frames each in MRC format]
Publication:

Cryo-electron microscopy: an introduction to the technique, and considerations when working to establish a national facility

Bhella D

Biophysical reviews 11 (2019) 515-519

PMID: 31359340

Related EMDB entry:
Deposited:
2020-03-09
Released:
2020-04-09
Last modified:
2022-01-31
Imageset size:
3.00 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
1. Frame aligned multi-frame movies of Lumazine Synthase imaged on a CryoARM300 and DE64
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
1198
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.597, 0.597)
Details:
Lumazine Synthase was expressed in E.coli and purified by immobilised metal affinity chromatography. Particles were frozen on C-flat R1.2/1.3 grids. Imaging was performed at 200 kX magnification on a JEOL CryoARM 300 equipped with a Direct Electron DE64 detector. Energy filtered images were collected with a slit-width of 20eV using JADAS. A single image was recorded per hole using electron counting with two times binning.
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