EMPIAR-10381 - Lipid-stabilised asymmetric open state of the 5-HT3A serotonin receptor [multiple data sets in MRC format]

EMPIAR-10381

Lipid-stabilised asymmetric open state of the 5-HT3A serotonin receptor [multiple data sets in MRC format]

Publication:

Asymmetric opening of the homopentameric 5-HT_3A serotonin receptor in lipid bilayers

Zhang Y, Dijkman PM, Zou R, Zandl-Lang M, Sanchez RM, Eckhardt-Strelau L, Köfeler H, Vogel H, Yuan S, Kudryashev M

Nature communications 12 (2021)

PMID: 33594077

DOI: 10.1038/s41467-021-21016-7

Related EMDB entry:

EMD-10691 EMD-10692 EMD-10693

Deposited:

2020-02-25

Released:

2021-01-22

Last modified:

2022-02-08

Imageset size:

6.43 TB

Imageset DOI:

10.6019/EMPIAR-10381

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

MRC movies (*_Fractions.mrc) in this entry were compressed by bzip2 (pbzip2)
by EMPIAR Japan to save space and bandwidth.

Some programs (e.g. RELION >= 4.0.1 and CryoSPARC) can process compressed movies without explicit
decompression. For other programs, movies have to be decompressed beforehand. In this case,
you might want to convert decompressed MRC movies into TIFF to save your local storage.

Original size:   16,534,928,529 kilobytes
Compressed size:  6,712,563,653 kilobytes

Contains:

micrographs - multiframe

Image Sets

1. Lipid-stabilised 5-HT3A serotonin receptor in asymmetric open state

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

2261

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(0.832, 0.832)

Details:

The particles are the refined particles in the final asymmetric open map. Name called -
251k_particles.mrcs
251k_particles.star
They are generated using relion_stack_create, taking the run_data.star as input. The particle stack mrcs file is in a single stack containing all refined 251k particles for the asymmetric open state map. The micrographs are raw data, without any realignment, of 69 raw movie frame (accumulating 30 electron per squared Angstrom using Falcon III camera in a ~32 second exposure.)

Files:

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Download a list of selected files.

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2. Lipid-stabilised 5-HT3A serotonin receptor in apo state forms (symmetric C5 apo and asymmetric C1 apo)

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

5053

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(0.832, 0.832)

Details:

The particles are the refined particles in the final asymmetric apo state map and symmetric apo state map. Names called -
(1) 152k_particles.mrcs and 152_particles.star for asymmetric apo (C1) state map;
(2) 238k_particles.mrcs and 238_particles.star for symmetric apo (C5) state map.
They are generated using relion_stack_create, taking the run_data.star for each job respectively as input. The two particle stack mrcs files are in a single stack containing all refined particles for the asymmetric apo state map and symmetric apo state map, accordingly.
The micrographs are raw data, without any realignment, of 69 raw movie frame (accumulating 30 electron per squared Angstrom using Falcon III camera in a ~32 second exposure.)

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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