EMPIAR-10380 - Single particle cryo electron microscopy of the human serotonin transporter bound with paroxetine and in complex with 8B6 Fab [multiple data sets in TIFF and MRC formats]

EMPIAR-10380

Single particle cryo electron microscopy of the human serotonin transporter bound with paroxetine and in complex with 8B6 Fab [multiple data sets in TIFF and MRC formats]

Publication:

Chemical and structural investigation of the paroxetine-human serotonin transporter complex

Coleman JA, Navratna V, Antermite D, Yang D, Bull JA, Gouaux E

eLife 9 (2020)

PMID: 32618269

DOI: 10.7554/elife.56427

Related EMDB entry:

EMD-21368

Deposited:

2020-02-10

Released:

2020-07-01

Last modified:

2022-01-14

Imageset size:

2.66 TB

Imageset DOI:

10.6019/EMPIAR-10380

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
micrographs - single frame
picked particles - multiframe - unprocessed

Image Sets

1. Dataset 1 unaligned multi-frame micrographs of SERT-8B6 Fab complex bound to paroxetine

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2557

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.324, 0.324)

Details:

Multiframe movies contain 40 raw frames with a total dose of 54 e-/A^2.

Files:

Archives and downloads the selected files into an uncompressed zip file.

Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

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Download a list of selected files.

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rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. Dataset 2 unaligned multi-frame micrographs of SERT-8B6 Fab complex bound to paroxetine

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4613

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.324, 0.324)

Details:

Multiframe movies contain 40 raw frames with a total dose of 60 e-/A^2.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Aligned micrographs of the SERT-8B6 paroxetine Fab complex bound to paroxetine

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

7170

Image size:

(5760, 4092)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.648, 0.648)

Details:

Aligned micrographs. Picked particles also follow pattern: data/coord/g3_F_paro_05702_mc_DW_coord.star

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

4. Particle stack of the SERT-8B6 paroxetine Fab complex bound to paroxetine

Category:

picked particles - multiframe - unprocessed

Image format:

MRC

No. of images or tilt series:

420373

Image size:

(400, 400)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.648, 0.648)

Details:

Final refined particle stack and coordinates. Picked particles also follow pattern: data/coord/g3_F_paro_05702_mc_DW_coord.star

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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