EMPIAR-10347
FAK structure from single particle analysis of 2D crystals [multiple data sets in MRC, TIFF and MRCS formats]
Publication:

Structural basis of Focal Adhesion Kinase activation on lipid membranes

Acebrón I, Righetto RD, Schoenherr C, de Buhr S, Redondo P, Culley J, Rodríguez CF, Daday C, Biyani N, Llorca O, Byron A, Chami M, Gräter F, Boskovic J, Frame MC, Stahlberg H, Lietha D

The EMBO journal (2020)

PMID: 32779739

Related EMDB entry:
Deposited:
2020-01-16
Released:
2020-08-25
Last modified:
2020-08-25
Imageset size:
1.79 TB
Imageset DOI:
Experimental metadata:
Contains:
  • micrographs - single frame
  • micrographs - multiframe
  • picked particles - single frame - unprocessed
1. Dose-weighted aligned movie averages of the FAK AMP-PNP dataset
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
2513
Image size:
(3838, 3710)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.058, 1.058)
Details:
The filenames contain the tilt angle estimated by FOCUS as well as their unique ID within the FOCUS project.
Micrographs have been downsampled by a factor of two in relation to the super-resolution movies, i.e. they are at the physical pixel size.
More details in the data/README file.
Files:
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2. Unaligned movies of the FAK AMP-PNP dataset
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
2513
Image size:
(7676, 7420)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.529, 0.529)
Details:
Movies have been acquired in super-resolution mode of the K2 detector.
The gain reference and defects list file are stored under data/amp-pnp/micrographs/gainref/.
The filenames contain the tilt angle estimated by FOCUS as well as their unique ID within the FOCUS project.
More details in the data/README file.
Files:
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3. Stack of particles extracted from the FAK AMP-PNP dataset
Category:
picked particles - single frame - unprocessed
Image format:
MRCS
No. of images or tilt series:
2666154
Image size:
(200, 200)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(2.116, 2.116)
Details:
Particle boxes have been downsampled by a factor of two in relation to the aligned micrographs.
The initial orientations and defocii values are determined from the 2D crystal processing are available in the particles.star file.
More details in the data/README file.
Files:
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4. Dose-weighted aligned movie averages of the FAK APO Polara dataset
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
88
Image size:
(3808, 3808)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.7, 1.7)
Details:
The filenames contain the tilt angle estimated by FOCUS as well as their unique ID within the FOCUS project.
More details in the data/README file.
Files:
Loading...
5. Dose-weighted aligned movie averages of the FAK APO Titan dataset
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
509
Image size:
(3808, 3808)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.058, 1.058)
Details:
The filenames contain the tilt angle estimated by FOCUS as well as their unique ID within the FOCUS project.
More details in the data/README file.
Files:
Loading...
6. Unaligned movies of the FAK APO Titan dataset
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
509
Image size:
(7676, 7420)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.529, 0.529)
Details:
Movies have been acquired in super-resolution mode of the K2 detector.
The gain reference and defects list file are stored under data/apo/micrographs/titan/gainref/.
The filenames contain the tilt angle estimated by FOCUS as well as their unique ID within the FOCUS project.
More details in the data/README file.
Files:
Loading...
7. Stack of particles extracted from the FAK APO Titan and Polara datasets
Category:
picked particles - single frame - unprocessed
Image format:
MRCS
No. of images or tilt series:
361796
Image size:
(238, 238)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.7, 1.7)
Details:
Particle boxes extracted from the Titan dataset have been downsampled to approximately match the pixel size of the Polara dataset. The stack contains all particles extracted from the FAK APO Titan and Polara datasets merged.
The initial orientations and defocii values are determined from the 2D crystal processing are available in the particles_preprocessed.star file.
More details in the data/README file.
Files:
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Files:
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Quick Links
Citations
Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)
Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)
Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)
Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)
See all citations
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