CryoEM dataset containing multiple conformations of the asymmetric αVβ8 integrin bound to latent TGF-β on a graphene oxide grid (preferred orientations) [multiple data sets in TIFF, MRC and MRCS formats]
Publication:
Cryo-EM Reveals Integrin-Mediated TGF-β Activation without Release from Latent TGF-β
αVβ8 integrin bound to latent TGF-β on graphene oxide grids (particles have preferred orientations): 2682 unaligned uncorrected movies were recorded automatically using SerialEM on a K2 camera in super-resolution (dstep=2.5) mode on a Titan Krios microscope operated at 300 kV (Cs=2.7) equipped with an energy filter using a slit width of 20 eV. The 16 second movies contain 80 frames and the super resolution pixel size is 0.6725 Å/px. The total dose per movie is ~70 e/Å^2. The gain reference, a file containing coordinates for a single dead column, and a list of the 2239 micrographs that were used for downstream processing are included.
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αVβ8 integrin bound to latent TGF-β on graphene oxide grids (particles have preferred orientations): 2682 aligned and dose-weighted (in MotionCor2) micrographs recorded automatically using SerialEM on a K2 camera (dstep=5) in super-resolution mode on a Titan Krios microscope operated at 300 kV (Cs=2.7) equipped with an energy filter using a slit width of 20 eV. Images were binned by 2 using Fourier cropping and the binned pixel size is 1.345. The total dose per micrograph is ~70 e/Å^2. A list of the 2239 micrographs that were used for downstream processing is included.
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αVβ8 integrin bound to latent TGF-β on graphene oxide grids (particles have preferred orientations): Stacks of 163,989 dose-weighted (in MotionCor2) particles contributing to the seven conformational snapshots described in the publication . Particles were selected after extensive 2D- and 3D classification. Images were recorded automatically using SerialEM on a K2 camera (dstep=5) in super-resolution mode on a Titan Krios microscope operated at 300 kV (Cs=2.7) equipped with an energy filter using a slit width of 20 eV. Images were binned by 2 using Fourier cropping and the binned pixel size is 1.345. This particle stack was combined with a stack of particles collected on holey carbon grids to complement preferred orientations. The holey carbon particle stack is deposited with the accession number EMPIAR-10343.
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Archives and downloads the selected files into an uncompressed zip file.
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