EMPIAR-10326 - Raw FIB SEM images of a parasitophorous vacuole containing Toxoplasma gondii ∆CAP parasites. [4490 micrographs in TIFF format]

EMPIAR-10326

Raw FIB SEM images of a parasitophorous vacuole containing Toxoplasma gondii ∆CAP parasites. [4490 micrographs in TIFF format]

Publication:

Differential requirements for cyclase-associated protein (CAP) in actin-dependent processes of Toxoplasma gondii

Hunt A, Russell MRG, Wagener J, Kent R, Carmeille R, Peddie CJ, Collinson L, Heaslip A, Ward GE, Treeck M

eLife 8 (2019)

PMID: 31577230

DOI: 10.7554/eLife.50598

Related EMDB entry:

Deposited:

2019-09-30

Released:

2019-10-07

Last modified:

2019-10-07

Imageset size:

273.82 GB

Imageset DOI:

10.6019/EMPIAR-10326

Experimental metadata:

Download xml json

Contains:

micrographs - single frame

Image Sets

1. Raw FIB SEM images of a parasitophorous vacuole containing Toxoplasma gondii ∆CAP parasites.

Category:

micrographs - single frame

Image format:

TIFF

No. of images or tilt series:

4490

Image size:

(5632, 5632)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(50.0, 50.0)

Details:

Image acquisition;
5 nm voxels: xy pixel set to 5 nm, FIB mill every 5 nm.
SEM: 1.5 kV with 1 nA current, 10 μs dwell time.
EsB detector grid voltage: 1200 V.
Ion beam milling: 30 kV, 700 pA.

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