EMPIAR-10294 - Single particle cryo-EM dataset of clathrin cages with phase flipping suitable for refinement [stack of 12785 particles in MRCS format]

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EMPIAR-10294

Single particle cryo-EM dataset of clathrin cages with phase flipping suitable for refinement [stack of 12785 particles in MRCS format]

Publication:

Cryo-EM of multiple cage architectures reveals a universal mode of clathrin self-assembly

Smith CJ, Morris KL, Jones JR, Halebian M, Wu S, Baker M, Armache JP, Avila Ibarra A, Sessions RB, Cameron AD, Cheng Y

Nature structural & molecular biology 26 (2019) 890-898

PMID: 31582853

DOI: 10.1038/s41594-019-0292-0

Related EMDB entry:

EMD-0114 EMD-0115 EMD-0118 EMD-0116 EMD-0120

Deposited:

2019-07-12

Released:

2019-10-04

Last modified:

2019-10-11

Imageset size:

11.92 GB

Imageset DOI:

10.6019/EMPIAR-10294

Experimental metadata:

Download xml json

Contains:

picked particles - single frame - processed

Image Sets

1. Clathrin cage particle images with phase flipping

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

12785

Image size:

(500, 500)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.705, 1.705)

Details:

Particle images for whole cages with phase flipping to have a tight box around the cage. Particle images are extracted from MotionCor2 dose weighted micrographs. Star files for the refinement of the five different cage types are found in data/starfile_whole_cage/*.star, each refinement uses symmetry for the respective cage type.

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