EMPIAR-10238 - CryoET of mouse protocadherin gamma B6 on membranes (with energy filter) [multiple data sets in MRC format]

EMPIAR-10238

CryoET of mouse protocadherin gamma B6 on membranes (with energy filter) [multiple data sets in MRC format]

Publication:

Visualization of clustered protocadherin neuronal self-recognition complexes

Brasch J, Goodman KM, Noble AJ, Rapp M, Mannepalli S, Bahna F, Dandey VP, Bepler T, Berger B, Maniatis T, Potter CS, Carragher B, Honig B, Shapiro L

Nature 569 (2019) 280-283

PMID: 30971825

DOI: 10.1038/s41586-019-1089-3

Related EMDB entry:

EMD-9199

Deposited:

2018-10-03

Released:

2019-04-18

Last modified:

2019-06-07

Imageset size:

411.33 GB

Imageset DOI:

10.6019/EMPIAR-10238

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
micrographs - single frame
tilt series

Image Sets

1. K2 tilt-series frames

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

Image size:

(3710, 3838)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.84, 1.84)

Details:

~8 e-/px/s dose rate on the camera
Gain and dark references included

Files:

Archives and downloads the selected files into an uncompressed zip file.

Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. Whole-frame aligned tilt images along with all other magnification images from the collection

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

Image size:

(3710, 3838)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.84, 1.84)

Details:

Whole-frame aligned tilt images using Motioncor2.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Appion-Protomo tilt-series alignments

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(3710, 3838)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.84, 1.84)

Details:

Includes a full tilt-series alignment run for each tilt-series.
To view properly, navigate to the protomo_alignments folder in Appion-Protomo.
The export/ directory contains SerialEM-formatted unaligned tilt-series stacks, mdoc files, and tilt files. This allows for easy upload to Appion-Protomo.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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