EMPIAR-10214
CryoET of bacterial RNA polymerase with several detergents [multiple data sets in MRC and RAW TEXT formats]
Publication:

Eliminating effects of particle adsorption to the air/water interface in single-particle cryo-electron microscopy: Bacterial RNA polymerase and CHAPSO

Chen J, Noble AJ, Kang JY, Darst SA

Journal of Structural Biology: X (2019)

Related EMDB entry:
Deposited:
2018-10-05
Released:
2018-10-25
Last modified:
2019-04-10
Imageset size:
207.49 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • micrographs - single frame
  • picked particles - multiframe - unprocessed
  • tilt series
1. K2 tilt-series frames
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
8
Image size:
(3710, 3838)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(1.33, 1.33)
Details:
~8 e-/px/s dose rate on the camera
Gain and dark references included
Files:
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2. Whole-frame aligned tilt images along with all other magnification images from the collection
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
17
Image size:
(3710, 3838)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.33, 1.33)
Details:
Whole-frame aligned tilt images using Motioncor2.
Files:
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3. Dynamo manual particle picks of tomograms
Category:
picked particles - multiframe - unprocessed
Image format:
RAW TEXT
No. of images or tilt series:
4
Image size:
(None, None)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(5.32, 5.32)
Details:
Manual particle picks of binned by 4 tomograms using Dynamo.
Files:
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4. Appion-Protomo tilt-series alignments
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
13
Image size:
(3710, 3838)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.33, 1.33)
Details:
Includes a full tilt-series alignment run for each tilt-series.
To view properly, navigate to the protomo_alignments folder in Appion-Protomo.
The export/ directory contains SerialEM-formatted unaligned tilt-series stacks, mdoc files, and tilt files. This allows for easy upload to Appion-Protomo.
Files:
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Files:
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