EMPIAR-10213
mouse MDA5-dsRNA Filaments in presence of 1mM AMPPNP [multiple data sets in MRC format]
Publication:

Cryo-EM Structures of MDA5-dsRNA Filaments at Different Stages of ATP Hydrolysis

Yu Q, Qu K, Modis Y.E

Molecular cell 72 (2018) 999-1012.e6

PMID: 30449722

Related EMDB entry:
Deposited:
2018-04-05
Released:
2019-04-05
Last modified:
2022-04-21
Imageset size:
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Imageset DOI:
Experimental metadata:
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Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

MRC movies (FoilHole*Fractions.mrc) in this entry were compressed by bzip2 (pbzip2)
by EMPIAR Japan to save space and bandwidth.

Some programs (e.g. RELION >= 4.0.1 and CryoSPARC) can process compressed movies without explicit
decompression. For other programs, movies have to be decompressed beforehand. In this case,
you might want to convert decompressed MRC movies into TIFF to save your local storage.

Original size:   3,737,344,892 bytes
Compressed size: 1,640,014,468 bytes
Contains:
  • micrographs - multiframe
1. mouse MDA5 and bacteriophage dsRNA filaments in complex with 1mM AMPPNP_data1
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
891
Image size:
(4096, 4096)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(1.07, 1.07)
Details:
Unaligned raw movies of mouse MDA5-dsRNA filaments in complex with 1mM AMPPNP
Total dose was 29.85 electrons Å -2 over 60 seconds. Calibrated pixel size is 1.07 Å/pixel.
Picked particles file are raw particles from RELION Autopick. Not final particles for reconstruction.
Files:
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2. Unaligned multi-frame movies of mouse MDA5-dsRNA filaments in complex with 1mM AMPPNP_data2
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
421
Image size:
(4096, 4096)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(1.07, 1.07)
Details:
Unaligned raw movies of mouse MDA5-dsRNA filaments in complex with 1mM AMPPNP.
Total dose was 29.85 electrons Å -2 over 60 seconds. Calibrated pixel size is 1.07 Å/pixel.
Picked particles file are raw particles from RELION Autopick. Not final particles for reconstruction.
Files:
Loading...
3. Unaligned multi-frame movies of mouse MDA5-dsRNA filaments in complex with 1mM AMPPNP_data3
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
210
Image size:
(4096, 4096)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(1.07, 1.07)
Details:
Unaligned raw movies of mouse MDA5-dsRNA filaments in complex with 1mM AMPPNP
Total dose was 29.85 electrons Å -2 over 60 seconds. Calibrated pixel size is 1.07 Å/pixel.
Picked particles file are raw particles from RELION Autopick. Not final particles for reconstruction.
Files:
Loading...
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