EMPIAR-10109 - FIB-SEM of a dividing cell at 11.2 min after anaphase onset [777 multi-frame micrographs composed of 1 frames each in TIFF format]

EMPIAR-10109

FIB-SEM of a dividing cell at 11.2 min after anaphase onset [777 multi-frame micrographs composed of 1 frames each in TIFF format]

Publication:

Postmitotic nuclear pore assembly proceeds by radial dilation of small ER membrane openings

Otsuka S, Steyer AM, Schorb M, Heriche JK, Hossain MJ, Sethi S, Kueblbeck M, Schwab Y, Beck M, Ellenberg J

Nature Structural & Molecular Biology (2017)

DOI: 10.1038/s41594-017-0001-9

Related EMDB entry:

Deposited:

2017-06-17

Released:

2017-11-30

Last modified:

2017-11-30

Imageset size:

10.96 GB

Imageset DOI:

10.6019/EMPIAR-10109

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. FIB-SEM images of a dividing cell at 11.2 min after anaphase onset

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

777

Image size:

(5064, 2992)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(5.0, 8.0)

Details:

The pixel size in xy was set to be 5 nm and the FIB-milling was done every 8 nm. The acquired raw FIB-SEM images were aligned using TrackEM2 (Cardona et al., PloS one, e38011, 2012) in ImageJ (http://rsbweb.nih.gov/ij/). After alignment, the stack was cropped, gray levels were inverted and the images were smoothened with a mean filter (kernel size: 15 × 15 nm). A small part of the nucleus is missing.

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Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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