EMPIAR-10097
40 Degree Tilted Single-Particle CryoEM of Highly Preferred Orientated Influenza Hemagglutinin Trimer [multiple data sets in MRC format]
Publication:

Addressing preferred specimen orientation in single-particle cryo-EM through tilting

Tan YZ, Baldwin PR, Davis JH, Williamson JR, Potter CS, Carragher B, Lyumkis D

Nature methods 14 (2017) 793-796

PMID: 28671674

Related EMDB entry:
Deposited:
2017-05-11
Released:
2017-08-16
Last modified:
2021-11-02
Imageset size:
1.80 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • micrographs - single frame
  • picked particles - multiframe - processed
1. Unaligned raw multi-frame 40 degree tilted micrographs of Influenza Hemagglutinin Trimer
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
668
Image size:
(3838, 3710)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.31, 1.31)
Details:
Unaligned raw multi-frame 40 degree tilted micrographs of Influenza Hemagglutinin Trimer. Micrographs were collected when tilt angle is set to -40 degrees. The gain normalization reference images (bright and norm) are included for each of the 3 sessions. No dark gain reference images are present because dark images values are always zero in counting mode camera.
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2. Aligned, summed and exposure weighted 40 degree tilted micrographs of Influenza Hemagglutinin Trimer
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
668
Image size:
(3838, 3710)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.31, 1.31)
Details:
Aligned, summed and exposure weighted 40 degree tilted micrographs of Influenza Hemagglutinin Trimer. Micrographs were aligned and summed from the raw frames using MotionCor2, with 5 by 5 patch and exposure weighting.
Files:
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3. Equalized particle stack of Influenza Hemagglutinin Trimer from 40 degree tilted micrographs
Category:
picked particles - multiframe - processed
Image format:
MRC
No. of images or tilt series:
130000
Image size:
(256, 256)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.31, 1.31)
Details:
Equalized particle stack of Influenza Hemagglutinin Trimer from 40 degree tilted micrographs. Particles were extracted from aligned, exposured weighted and summed micrographs. In order to compare these particles with a 40 degree tilted dataset, these particles were equalized based on GCTF-estimated per-particle CTF values so a subset of particles with the same defocus spread would be obtained. This equalized stack of 130,000 is the uploaded stack. A Relion .star metadata file is also provided containing the final refined Euler angles.
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