EMPIAR-10072 - Structural basis for dynamic regulation of the human 26S proteasome [multiple data sets in SPIDER and MRC formats]

EMPIAR-10072

Structural basis for dynamic regulation of the human 26S proteasome [multiple data sets in SPIDER and MRC formats]

Publication:

Structural basis for dynamic regulation of the human 26S proteasome

Chen S, Wu J

Proceedings of the National Academy of Sciences of the United States of America 113 (2016) 12991-12996

PMID: 27791164

DOI: 10.1073/pnas.1614614113

Related EMDB entry:

EMD-8337 EMD-8332 EMD-8335 EMD-8333 EMD-8334 EMD-8336

Deposited:

2016-08-17

Released:

2016-11-10

Last modified:

2016-11-10

Imageset size:

2.01 TB

Imageset DOI:

10.6019/EMPIAR-10072

Experimental metadata:

Download xml json

Contains:

micrographs - single frame
picked particles - multiframe - processed

Image Sets

1. Drift-corrected micrographs of human 26S proteasome holoenzyme

Category:

micrographs - single frame

Image format:

SPIDER

No. of images or tilt series:

6967

Image size:

(7420, 7676)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.86, 0.86)

Details:

The micrograph are the average from 30 re-aligned movie frames to minimize any effect of drift or sample movement, accumulating 30 electron per squared Angstrom in a 6 second exposure.

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2. Classified particle datasets for the human proteasome in four conformational states

Category:

picked particles - multiframe - processed

Image format:

MRC

No. of images or tilt series:

506186

Image size:

(None, None)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(None, None)

Details:

The description of particle stack: (1) 26S.mrcs 237,083 particles for double cap with dimension of 640*640. (2) 26S_A.mrcs 85,420 particles for double cap at SA state, the dimension of particles is 640*640; (3) 26SH_A.mrcs 139,236 particles for RP-CP complex at SA state, the dimension of particles is 360*360; (4) 26SH_B.mrcs 18,443 particles for RP-CP complex at SB state, the dimension of particles is 360*360; (5) 26SH_C.mrcs 10,622 particles for RP-CP complex at SC state, the dimension of particles is 360*360; (6) 26SH_D.mrcs 14,382 particles for RP-CP complex at SD state, the dimension of particles is 360*360; the pixel size of all the particles stack is 0.86 angstrom.

Files:

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We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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