EMPIAR-10070
Focused Ion Beam-Scanning Electron Microscopy of mitochondrial reticulum in murine skeletal muscle [291 reconstructed volumes in MRC format]
Publication:

Mitochondrial reticulum for cellular energy distribution in muscle

Glancy B, Hartnell LM, Malide D, Yu ZX, Combs CA, Connelly PS, Subramaniam S, Balaban RS

Nature 523 (2015) 617-620

PMID: 26223627

Related EMDB entry:
Deposited:
2016-11-16
Released:
2016-11-18
Last modified:
2023-07-07
Imageset size:
1.47 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • reconstructed volumes
1. aligned stack: FIB-SEM volume through skeletal muscle tissue
Category:
reconstructed volumes
Image format:
MRC
No. of images or tilt series:
291
Image size:
(1675, 1595)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(50.0, 50.0)
Details:
MRC is of an aligned stack of tiffs that are binned by 3 in x and y. Z thickness is ~15 nm.

Volume rendered components found in Figures 1 (paravascular region in murine skeletal muscle) and Figure 2 (skeletal muscle mitochondria morphologies, nucleus, and blood vessel), Glancy et al: http://www.nature.com/nature/journal/v523/n7562/pdf/nature14614.pdf are located in respective Figure folders. Software used for rendering/segmentation: ImageJ/TRAKEM2 plugin.
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