EMPIAR-10070 - Focused Ion Beam-Scanning Electron Microscopy of mitochondrial reticulum in murine skeletal muscle [291 reconstructed volumes in MRC format]

EMPIAR-10070

Focused Ion Beam-Scanning Electron Microscopy of mitochondrial reticulum in murine skeletal muscle [291 reconstructed volumes in MRC format]

Publication:

Mitochondrial reticulum for cellular energy distribution in muscle

Glancy B, Hartnell LM, Malide D, Yu ZX, Combs CA, Connelly PS, Subramaniam S, Balaban RS

Nature 523 (2015) 617-620

PMID: 26223627

DOI: 10.1038/nature14614

Related EMDB entry:

Deposited:

2016-11-16

Released:

2016-11-18

Last modified:

2023-07-07

Imageset size:

1.47 GB

Imageset DOI:

10.6019/EMPIAR-10070

Experimental metadata:

Download xml json

Contains:

reconstructed volumes

Image Sets

1. aligned stack: FIB-SEM volume through skeletal muscle tissue

Category:

reconstructed volumes

Image format:

MRC

No. of images or tilt series:

291

Image size:

(1675, 1595)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(50.0, 50.0)

Details:

MRC is of an aligned stack of tiffs that are binned by 3 in x and y. Z thickness is ~15 nm.

Volume rendered components found in Figures 1 (paravascular region in murine skeletal muscle) and Figure 2 (skeletal muscle mitochondria morphologies, nucleus, and blood vessel), Glancy et al: http://www.nature.com/nature/journal/v523/n7562/pdf/nature14614.pdf are located in respective Figure folders. Software used for rendering/segmentation: ImageJ/TRAKEM2 plugin.

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