EMPIAR-10059 - Structure of TRPV1 in complex with DkTx and RTX, determined in lipid nanodisc [multiple data sets in MRC format]

EMPIAR-10059

Structure of TRPV1 in complex with DkTx and RTX, determined in lipid nanodisc [multiple data sets in MRC format]

Publication:

TRPV1 structures in nanodiscs reveal mechanisms of ligand and lipid action

Gao Y, Cao E, Julius D, Cheng Y

Nature 534 (2016) 347-351

PMID: 27281200

DOI: 10.1038/nature17964

Related EMDB entry:

EMD-8117

Deposited:

2016-06-07

Released:

2016-06-07

Last modified:

2016-06-07

Imageset size:

100.67 GB

Imageset DOI:

10.6019/EMPIAR-10059

Experimental metadata:

Download xml json

Contains:

micrographs - single frame
picked particles - multiframe - unprocessed

Image Sets

1. Motion corrected, dose-weighted sum of micrographs of TRPV1-DkTx/RTX embedded in lipid nanodisc

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

1200

Image size:

(3838, 3710)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.2156, 1.2156)

Details:

All image stacks (accumulating 41 electrons per squared Angstrom exposure in 30 frames / 6 second exposure) were drift-corrected using software UcsfDfCorr. '5 X 5' patch option was used. Dose rate was set to 1.4 electrons to perform the exposure filtering procedure implemented in the program. Deposited data is the sum of all corrected frames.

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2. Raw particle image stack of TRPV1-DkTx/RTX embedded in lipid nanodisc

Category:

picked particles - multiframe - unprocessed

Image format:

MRC

No. of images or tilt series:

218805

Image size:

(192, 192)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.2156, 1.2156)

Details:

Particle image stacks extracted from individual micrographs, without further processing.
'particles.star' list all relevant information for every particle in all image stacks (coordinate, defocus etc.)

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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