EMPIAR-10044 - In vitro assembled bacteriophage phi6 polymerase complex [stack of 798 particles in MRC format]

EMPIAR-10044

In vitro assembled bacteriophage phi6 polymerase complex [stack of 798 particles in MRC format]

Publication:

Localized reconstruction of subunits from electron cryomicroscopy images of macromolecular complexes

Ilca SL, Kotecha A, Sun X, Poranen MP, Stuart DI, Huiskonen JT

Nat Commun 6 (2015) 8843

PMID: 26534841

DOI: doi:10.1038/ncomms9843

Related EMDB entry:

EMD-3187

Deposited:

2015-10-30

Released:

2015-11-04

Last modified:

2015-11-04

Imageset size:

8.05 GB

Imageset DOI:

10.6019/EMPIAR-10044

Experimental metadata:

Download xml json

Contains:

picked particles - multiframe - processed

Image Sets

1. Extracted particle stacks of bacteriophage phi6 polymerase complex

Category:

picked particles - multiframe - processed

Image format:

MRC

No. of images or tilt series:

798

Image size:

(480, 480)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.35, 1.35)

Details:

Particle extracted and normalized in Relion. Contrast has been inverted. Each of 798 MRCS files is a stack of individual particle images (not multiple frames of the same particle image).

Files:

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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