EMPIAR-10017 - Beta-galactosidase Falcon-II micrographs plus manually selected coordinates by Richard Henderson [84 micrographs in MRC format]

EMPIAR-10017

Beta-galactosidase Falcon-II micrographs plus manually selected coordinates by Richard Henderson [84 micrographs in MRC format]

Publication:

Semi-automated selection of cryo-EM particles in RELION-1.3.

Scheres SH

J Struct Biol 189 (2015) 114-122

PMID: 25486611

DOI: doi:10.1016/j.jsb.2014.11.010

Related EMDB entry:

EMD-2824

Deposited:

2014-11-19

Released:

2014-11-19

Last modified:

2014-11-19

Imageset size:

5.25 GB

Imageset DOI:

10.6019/EMPIAR-10017

Experimental metadata:

Download xml json

Version history:

2015-10-28

REL

Restructured the entry directory to have "data" and ".private".

Contains:

micrographs - single frame

Image Sets

1. Beta-galactosidase micrographs + coordinates

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.77, 1.77)

Details:

Particle positions in corresponding .coord text files (with X and Y coordinates for manually selected coordinates in 1st and 2nd column respectively).
The micrographs are the average, without any realignment, of 24 raw movie frames (accumulating 24 electron per squared Angstrom in a 1.5 second exposure).

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Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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