EMPIAR-12810 - 70S initiation complex (tRNA-fMet M1 + GUG start codon) [2440 multi-frame micrographs composed of 48 frames each in TIFF format]

EMPIAR-12810

70S initiation complex (tRNA-fMet M1 + GUG start codon) [2440 multi-frame micrographs composed of 48 frames each in TIFF format]

Publication:

Structural analysis of noncanonical translation initiation complexes

Mattingly JM, Nguyen HA, Roy B, Fredrick K, Dunham CM

Journal of Biological Chemistry 300 (2024) 107743-107743

PMID: 39222680

DOI: 10.1016/j.jbc.2024.107743

Related EMDB entry:

EMD-45572

Deposited:

2025-06-04

Released:

2025-07-30

Last modified:

2025-07-30

Imageset size:

705.69 GB

Imageset DOI:

10.6019/EMPIAR-12810

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame movies of 70S initiation complex containing fMet-tRNA-fMet and a GUG start codon

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2440

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.045, 1.045)

Details:

Micrographs are 48-frame movies with a pixel size of 1.045 angstroms and an accumulated dose of 58.4 electrons per square angstrom. Files in the included "xml" folder contain Thermo EPU-format image shift data which can be used for beam shift group splitting. A gain reference file is included as "gain.mrc".

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Citations

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Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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