EMPIAR-12796 - CryoEM dataset of Retron-Eco1 E106A filaments [7748 multi-frame micrographs composed of 40 frames each in MRC format]

EMPIAR-12796

CryoEM dataset of Retron-Eco1 E106A filaments [7748 multi-frame micrographs composed of 40 frames each in MRC format]

Publication:

Retron-Eco1 assembles NAD⁺-hydrolyzing filaments that provide immunity against bacteriophages

Carabias A, Camara-Wilpert S, Mestre MR, Lopéz-Méndez B, Hendriks IA, Zhao R, Pape T, Fuglsang A, Luk SH, Nielsen ML, Pinilla-Redondo R, Montoya G

Molecular cell 84 (2024) 2185-2202000000000000.0

PMID: 38788717

DOI: 10.1016/j.molcel.2024.05.001

Related EMDB entry:

Deposited:

2025-05-26

Released:

2025-07-01

Last modified:

2025-07-01

Imageset size:

9.72 TB

Imageset DOI:

10.6019/EMPIAR-12796

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multiframe micrographs of Retron-Eco1 E106A mutant

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

7748

Image size:

(4096, 4096)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.832, 0.832)

Details:

Movies were collected in a Titan Krios G2 equipped with a TFS Falcon III camera operated at 300 keV in counting mode at a magnification of x 96000 (0.832 A˚ /px) using a total dose of 41 e/A˚ 2. Spherical Aberration: 2.7 mm. Out of the original 7748 micrographs, 7250 were used for particle picking. Micrographs are gain-corrected, non-motion-corrected.

Microscope Settings:
-Acceleration Voltage: 300 kV
-Pixel Size: 0.832 Å
-Spherical Aberration: 2.7 mm
-Total Exposure Dose: 41 e/Å²
-Number of Fractions: 40

The images for every grid square are in different directories following the next architecture:

data/GM_artcar-acdr0052_86_Filament_20220720_094043/Images-Disc1/GridSquare_*/Data

Files:

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

See all citations

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