EMPIAR-12741
CryoEM movies of human methemoglobin (metHb) [multiple data sets in EER and MRC formats]
Publication:

Preparation of oxygen-sensitive proteins for high-resolution cryoEM structure determination using blot-free vitrification

Cook BD, Narehood SM, McGuire KL, Li Y, Akif Tezcan F, Herzik Jr MA

Nature communications 16 (2025) 3528-3528

PMID: 40229244

Related EMDB entry:
Deposited:
2025-04-24
Released:
2025-06-26
Last modified:
2025-06-26
Imageset size:
2.13 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • micrographs - single frame
1. Unaligned multi-frame micrographs of human methemoglobin (metHb)
Category:
micrographs - multiframe
Image format:
EER
No. of images or tilt series:
2789
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.735, 0.735)
Details:
Unaligned multi-frame micrographs of human methemoglobin (metHb) collected on a Titan Krios G4 operating at 300 kV equipped with a Selectris-X energy filter with a 10eV slit width. All images were collected at a nominal magnification of 165,000x in EF-TEM mode (with a calibrated pixel size of 0.735 Å) on a Falcon4 direct electron detector. Micrographs were acquired in EER format with a cumulative electron exposure of 60 electrons/Å2.
Files:
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2. Aligned micrographs of human methemoglobin (metHb)
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
2789
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.735, 0.735)
Details:
Aligned micrographs of human methemoglobin (metHb) collected on a Titan Krios G4 operating at 300 kV equipped with a Selectris-X energy filter with a 10eV slit width. All images were collected at a nominal magnification of 165,000x in EF-TEM mode (with a calibrated pixel size of 0.735 Å) on a Falcon4 direct electron detector. Micrographs were acquired in EER format with a cumulative electron exposure of 60 electrons/Å2.
Files:
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3. Gain file for motion correction of unaligned micrographs of human methemoglobin (metHb)
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
1
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.735, 0.735)
Details:
Gain file required for motion correction of unaligned micrographs of human methemoglobin (metHb).
Files:
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